Molecular studies in olive (Olea europaea L.): overview on DNA markers applications and recent advances in genome analysis.

Olive (Olea europaea L.) is one of the oldest agricultural tree crops worldwide and is an important source of oil with beneficial properties for human health.\r\nThis emblematic tree crop of the Mediterranean Basin, which has conserved a very wide germplasm estimated in more than 1,200 cultivars, is a diploid species (2n = 2x = 46) that is present in two forms, namely wild (Olea europaea subsp. europaea var. sylvestris) and cultivated\r\n(Olea europaea subsp. europaea var. europaea). In spite of its economic and nutritional importance, there are few data about the genetic of olive if compared with other fruit crops. Available molecular data are especially related to the application of molecular markers to the analysis of genetic variability in Olea europaea complex and to develop efficient molecular tools for the olive oil origin\r\ntraceability. With regard to genomic research, in the last years efforts are made for the identification of expressed\r\nsequence tag, with particular interest in those sequences expressed during fruit development and in pollen allergens.\r\nVery recently the sequencing of chloroplast genome provided new information on the olive nucleotide sequence,opening the olive genomic era. In this article, we provide an overview of the most relevant results in olive molecular studies. A particular attention was given to DNA markers\r\nand their application that constitute the most part of published researches. The first important results in genome\r\nanalysis were reported.