TY - JOUR
T1 - Modulation of microbial consortia enriched from different polluted environments during petroleum biodegradation
AU - Omrani, Rahma
AU - Spini, Giulia
AU - Puglisi, Edoardo
AU - Saidane, Dalila
PY - 2018
Y1 - 2018
N2 - Environmental microbial communities are key players in the bioremediation of hydrocarbon pollutants. Here we assessed changes in bacterial abundance and diversity during the degradation of Tunisian Zarzatine oil by four indigenous bacterial consortia enriched from a petroleum station soil, a refinery reservoir soil, a harbor sediment and seawater. The four consortia were found to efficiently degrade up to 92.0% of total petroleum hydrocarbons after 2 months of incubation. Illumina 16S rRNA gene sequencing revealed that the consortia enriched from soil and sediments were dominated by species belonging to Pseudomonas and Acinetobacter genera, while in the seawater-derived consortia Dietzia, Fusobacterium and Mycoplana emerged as dominant genera. We identified a number of species whose relative abundances bloomed from small to high percentages: Dietzia daqingensis in the seawater microcosms, and three OTUs classified as Acinetobacter venetianus in all two soils and sediment derived microcosms. Functional analyses on degrading genes were conducted by comparing PCR results of the degrading genes alkB, ndoB, cat23, xylA and nidA1 with inferences obtained by PICRUSt analysis of 16S amplicon data: the two data sets were partly in agreement and suggest a relationship between the catabolic genes detected and the rate of biodegradation obtained. The work provides detailed insights about the modulation of bacterial communities involved in petroleum biodegradation and can provide useful information for in situ bioremediation of oil-related pollution.
AB - Environmental microbial communities are key players in the bioremediation of hydrocarbon pollutants. Here we assessed changes in bacterial abundance and diversity during the degradation of Tunisian Zarzatine oil by four indigenous bacterial consortia enriched from a petroleum station soil, a refinery reservoir soil, a harbor sediment and seawater. The four consortia were found to efficiently degrade up to 92.0% of total petroleum hydrocarbons after 2 months of incubation. Illumina 16S rRNA gene sequencing revealed that the consortia enriched from soil and sediments were dominated by species belonging to Pseudomonas and Acinetobacter genera, while in the seawater-derived consortia Dietzia, Fusobacterium and Mycoplana emerged as dominant genera. We identified a number of species whose relative abundances bloomed from small to high percentages: Dietzia daqingensis in the seawater microcosms, and three OTUs classified as Acinetobacter venetianus in all two soils and sediment derived microcosms. Functional analyses on degrading genes were conducted by comparing PCR results of the degrading genes alkB, ndoB, cat23, xylA and nidA1 with inferences obtained by PICRUSt analysis of 16S amplicon data: the two data sets were partly in agreement and suggest a relationship between the catabolic genes detected and the rate of biodegradation obtained. The work provides detailed insights about the modulation of bacterial communities involved in petroleum biodegradation and can provide useful information for in situ bioremediation of oil-related pollution.
KW - Bacterial communities
KW - Biodegradation
KW - Bioengineering
KW - Enrichment
KW - Environmental Chemistry
KW - Environmental Engineering
KW - Microbiology
KW - Next generation sequencing
KW - Petroleum hydrocarbons
KW - Pollution
KW - Bacterial communities
KW - Biodegradation
KW - Bioengineering
KW - Enrichment
KW - Environmental Chemistry
KW - Environmental Engineering
KW - Microbiology
KW - Next generation sequencing
KW - Petroleum hydrocarbons
KW - Pollution
UR - https://publicatt.unicatt.it/handle/10807/116421
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85042589694&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042589694&origin=inward
U2 - 10.1007/s10532-018-9823-3
DO - 10.1007/s10532-018-9823-3
M3 - Article
SN - 0923-9820
VL - 29
SP - 187
EP - 209
JO - Biodegradation
JF - Biodegradation
IS - 2
ER -