Modification of capillary electrophoresis selectivity in hydro-organic solutions - Dissociation constants and Stokes radius measurements of peptides in water-2,2,2-trifluoroethanol mixtures

Massimo Castagnola, D Rossetti, F Misiti, L Cassiano, B Giardina, I. Messana

Risultato della ricerca: Contributo in rivistaArticolo in rivista

19 Citazioni (Scopus)

Abstract

Peptide electrophoretic mobilities were measured at different acidic pH in water-2,2,2-trifluoroethanol mixtures. Fit of experimental mobilities according to binding equations allowed the calculation of peptide C-terminus dissociation constants and the Stokes radius of the differently protonated forms. Analysis of the data showed that the use of hydro-organic solvents in capillary electrophoresis offers the following principal advantages: (a) a modification of dissociation constants and Stokes radii that are strongly dependent upon peptide sequence and that can be conveniently utilised for selectivity manipulation; (b) an increase of separation performance arising from the stabilisation of particular peptide conformations; (c) a solubility of large apolar peptides with respect to aqueous solutions. (C) 1997 Published by Elsevier Science B.V.
Lingua originaleEnglish
pagine (da-a)57-65
Numero di pagine9
RivistaJOURNAL OF CHROMATOGRAPHY A
Volume792
DOI
Stato di pubblicazionePubblicato - 1997

Keywords

  • CAPILLARY

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