TY - JOUR
T1 - Milk microbiome diversity and bacterial group prevalence in a comparison between healthy holstein friesian and rendena cows
AU - Cremonesi, Paola
AU - Ceccarani, Camilla
AU - Curone, Giulio
AU - Severgnini, Marco
AU - Pollera, Claudia
AU - Bronzo, Valerio
AU - Riva, Federica
AU - Addis, Maria Filippa
AU - Filipe, Joel
AU - Amadori, Massimo
AU - Trevisi, Erminio
AU - Vigo, Daniele
AU - Moroni, Paolo
AU - Castiglioni, Bianca
PY - 2018
Y1 - 2018
N2 - Dry and early lactation periods represent the most critical phases for udder health in cattle, especially in highly productive breeds, such as the Holstein Friesian (HF). On the other hand, some autochthonous cattle breeds, such as the Rendena (REN), have a lower prevalence of mastitis and other transition-related diseases. In this study, milk microbiota of 6 HF and 3 REN cows, all raised on the same farm under the same conditions, was compared. A special focus was placed on the transition period to define bacterial groups’ prevalence with a plausible effect on mammary gland health. Four time points (dry-off, 1 d, 7–10 d and 30 d after calving) were considered. Through 16S rRNA sequencing, we characterized the microbiota composition for 117 out of the 144 milk samples initially collected, keeping only the healthy quarters, in order to focus on physiological microbiome changes and avoid shifts due to suspected diseases. Microbial populations were very different in the two breeds along all the time points, with REN milk showing a significantly lower microbial biodiversity. The taxonomic profiles of both cosmopolitan and local breeds were dominated by Firmicutes, mostly represented by the Streptococcus genus, although in very different proportions (HF 27.5%, REN 68.6%). Large differences in HF and REN cows were, also, evident from the metabolic predictive analysis from microbiome data. Finally, only HF milk displayed significant changes in the microbial composition along the transition period, while REN maintained a more stable microbiota. In conclusion, in addition to the influence on the final characteristics of dairy products obtained from milk of the two breeds, differences in the milk microbiome might, also, have an impact on their mammary gland health.
AB - Dry and early lactation periods represent the most critical phases for udder health in cattle, especially in highly productive breeds, such as the Holstein Friesian (HF). On the other hand, some autochthonous cattle breeds, such as the Rendena (REN), have a lower prevalence of mastitis and other transition-related diseases. In this study, milk microbiota of 6 HF and 3 REN cows, all raised on the same farm under the same conditions, was compared. A special focus was placed on the transition period to define bacterial groups’ prevalence with a plausible effect on mammary gland health. Four time points (dry-off, 1 d, 7–10 d and 30 d after calving) were considered. Through 16S rRNA sequencing, we characterized the microbiota composition for 117 out of the 144 milk samples initially collected, keeping only the healthy quarters, in order to focus on physiological microbiome changes and avoid shifts due to suspected diseases. Microbial populations were very different in the two breeds along all the time points, with REN milk showing a significantly lower microbial biodiversity. The taxonomic profiles of both cosmopolitan and local breeds were dominated by Firmicutes, mostly represented by the Streptococcus genus, although in very different proportions (HF 27.5%, REN 68.6%). Large differences in HF and REN cows were, also, evident from the metabolic predictive analysis from microbiome data. Finally, only HF milk displayed significant changes in the microbial composition along the transition period, while REN maintained a more stable microbiota. In conclusion, in addition to the influence on the final characteristics of dairy products obtained from milk of the two breeds, differences in the milk microbiome might, also, have an impact on their mammary gland health.
KW - Agricultural and Biological Sciences (all)
KW - Biochemistry, Genetics and Molecular Biology (all)
KW - Agricultural and Biological Sciences (all)
KW - Biochemistry, Genetics and Molecular Biology (all)
UR - http://hdl.handle.net/10807/126953
UR - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0205054&type=printable
U2 - 10.1371/journal.pone.0205054
DO - 10.1371/journal.pone.0205054
M3 - Article
SN - 1932-6203
VL - 13
SP - N/A-N/A
JO - PLoS One
JF - PLoS One
ER -