TY - JOUR
T1 - MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs
AU - Catanzaro, Giuseppina
AU - Besharat, Zein Mersini
AU - Garg, Neha
AU - Ronci, Maurizio
AU - Pieroni, Luisa
AU - Miele, Evelina
AU - Mastronuzzi, Angela
AU - Carai, Andrea
AU - Alfano, Vincenzo
AU - Po, Agnese
AU - Screpanti, Isabella
AU - Locatelli, Franco
AU - Urbani, Andrea
AU - Ferretti, Elisabetta
PY - 2016
Y1 - 2016
N2 - Medulloblastoma (MB) is the most common malignant brain tumor of pediatric age and is characterized by cells expressing stem, astroglial, and neuronal markers. Among them, stem-like cells (hMB-SLCs) represent a fraction of the tumor cell population with the potential of self-renewal and proliferation and have been associated with tumor poor prognosis. In this context, microRNAs have been described as playing a pivotal role in stem cells differentiation. In our paper, we analyze microRNAs profile and genes expression of hMB-SLCs before and after Retinoic Acid- (RA-) induced differentiation. We aimed to identify pivotal players of specific pathways sustaining stemness and/or tumor development and progression and integrate the results of our recent proteomic study. Our results uncovered 22 differentially expressed microRNAs that were used as input together with deregulated genes and proteins in the Genomatix Pathway System (GePS) analysis revealing 3 subnetworks that could be interestingly involved in the maintenance of hMB-SLCs proliferation. Taken together, our findings highlight microRNAs, genes, and proteins that are significantly modulated in hMB-SLCs with respect to their RA-differentiated counterparts and could open new perspectives for prognostic and therapeutic intervention on MB.
AB - Medulloblastoma (MB) is the most common malignant brain tumor of pediatric age and is characterized by cells expressing stem, astroglial, and neuronal markers. Among them, stem-like cells (hMB-SLCs) represent a fraction of the tumor cell population with the potential of self-renewal and proliferation and have been associated with tumor poor prognosis. In this context, microRNAs have been described as playing a pivotal role in stem cells differentiation. In our paper, we analyze microRNAs profile and genes expression of hMB-SLCs before and after Retinoic Acid- (RA-) induced differentiation. We aimed to identify pivotal players of specific pathways sustaining stemness and/or tumor development and progression and integrate the results of our recent proteomic study. Our results uncovered 22 differentially expressed microRNAs that were used as input together with deregulated genes and proteins in the Genomatix Pathway System (GePS) analysis revealing 3 subnetworks that could be interestingly involved in the maintenance of hMB-SLCs proliferation. Taken together, our findings highlight microRNAs, genes, and proteins that are significantly modulated in hMB-SLCs with respect to their RA-differentiated counterparts and could open new perspectives for prognostic and therapeutic intervention on MB.
KW - CANCER STEM-CELLS
KW - CYCLIN-E
KW - CANCER STEM-CELLS
KW - CYCLIN-E
UR - http://hdl.handle.net/10807/94153
U2 - 10.1155/2016/2683042
DO - 10.1155/2016/2683042
M3 - Article
SN - 1687-9678
VL - 2016
SP - 1
EP - 10
JO - Stem Cells International
JF - Stem Cells International
ER -
