MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis

Gabriele Di Sante, Elisa Gremese, Stefano Alivernini, Barbara Tolusso, Luca Petricca, Clara Di Mario, Gianfranco Ferraccioli, Mariola Kurowska-Stolarska, Emma Garcia Melchor, Aziza Elmesmari, Clare Tange, Derek S. Gilchrist, Chantal Keijzer, Lynn Stewart, Vicky Morrison, James M. Brewer, Duncan Porter, Simon Milling, Ronald D. Baxter, David MccareyGreg Lemke, Charles Mcsharry, Iain B. Mcinnes

Risultato della ricerca: Contributo in rivistaArticolo in rivista

42 Citazioni (Scopus)

Abstract

Current treatments for rheumatoid arthritis (RA) do not reverse underlying aberrant immune function. A genetic predisposition to RA, such as HLA-DR4 positivity, indicates that dendritic cells (DC) are of crucial importance to pathogenesis by activating auto-reactive lymphocytes. Here we show that microRNA-34a provides homoeostatic control of CD1c + DC activation via regulation of tyrosine kinase receptor AXL, an important inhibitory DC auto-regulator. This pathway is aberrant in CD1c + DCs from patients with RA, with upregulation of miR-34a and lower levels of AXL compared to DC from healthy donors. Production of pro-inflammatory cytokines is reduced by ex vivo gene-silencing of miR-34a. miR-34a-deficient mice are resistant to collagen-induced arthritis and interaction of DCs and T cells from these mice are reduced and do not support the development of Th17 cells in vivo. Our findings therefore show that miR-34a is an epigenetic regulator of DC function that may contribute to RA.
Lingua originaleEnglish
pagine (da-a)15877-15891
Numero di pagine15
RivistaNature Communications
Volume8
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • Biochemistry, Genetics and Molecular Biology (all)
  • Chemistry (all)
  • Physics and Astronomy (all)

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