MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis

Stefano Alivernini; Mariola Kurowska-Stolarska; Barbara Tolusso; Roberta Benvenuto; Aziza Elmesmari; Silvia Canestri; Luca Petricca; Antonella Mangoni; Anna Laura Fedele; Clara Di Mario; Maria Rita Gigante; Elisa Gremese; Iain B. Mcinnes; Gianfranco Ferraccioli

doi:10.1038/ncomms12970

MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis

Stefano Alivernini, Mariola Kurowska-Stolarska, Barbara Tolusso, Roberta Benvenuto, Aziza Elmesmari, Silvia Canestri, Luca Petricca, Antonella Mangoni, Anna Laura Fedele, Clara Di Mario, Maria Rita Gigante, Elisa Gremese, Iain B. Mcinnes, Gianfranco Ferraccioli

University of Glasgow

Risultato della ricerca: Contributo in rivista › Articolo in rivista

63 Citazioni (Scopus)

Abstract

MicroRNA-155 (miR-155) is an important regulator of B cells in mice. B cells have a critical role in the pathogenesis of rheumatoid arthritis (RA). Here we show that miR-155 is highly expressed in peripheral blood B cells from RA patients compared with healthy individuals, particularly in the IgD - CD27 - memory B-cell population in ACPA + RA. MiR-155 is highly expressed in RA B cells from patients with synovial tissue containing ectopic germinal centres compared with diffuse synovial tissue. MiR-155 expression is associated reciprocally with lower expression of PU.1 at B-cell level in the synovial compartment. Stimulation of healthy donor B cells with CD40L, anti-IgM, IL-21, CpG, IFN-α, IL-6 or BAFF induces miR-155 and decreases PU.1 expression. Finally, inhibition of endogenous miR-155 in B cells of RA patients restores PU.1 and reduces production of antibodies. Our data suggest that miR-155 is an important regulator of B-cell activation in RA.

Lingua originale	English
pagine (da-a)	12970-N/A
Rivista	Nature Communications
Volume	7
DOI	https://doi.org/10.1038/ncomms12970
Stato di pubblicazione	Pubblicato - 2016

Keywords

Arthritis
B cells
Comorbidities
Diagnosis algorithm
Psoriasis
Psoriatic arthritis
Rheumatology
miRNA

Accesso al documento

10.1038/ncomms12970

Altri file e collegamenti

Cita questo

Alivernini, S., Kurowska-Stolarska, M., Tolusso, B., Benvenuto, R., Elmesmari, A., Canestri, S., Petricca, L., Mangoni, A., Fedele, A. L., Di Mario, C., Gigante, M. R., Gremese, E., Mcinnes, I. B., & Ferraccioli, G. (2016). MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis. Nature Communications, 7, 12970-N/A. https://doi.org/10.1038/ncomms12970

@article{e0fbfb6ef61944c3ab472b1f9bc7f17b,

title = "MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis",

abstract = "MicroRNA-155 (miR-155) is an important regulator of B cells in mice. B cells have a critical role in the pathogenesis of rheumatoid arthritis (RA). Here we show that miR-155 is highly expressed in peripheral blood B cells from RA patients compared with healthy individuals, particularly in the IgD - CD27 - memory B-cell population in ACPA + RA. MiR-155 is highly expressed in RA B cells from patients with synovial tissue containing ectopic germinal centres compared with diffuse synovial tissue. MiR-155 expression is associated reciprocally with lower expression of PU.1 at B-cell level in the synovial compartment. Stimulation of healthy donor B cells with CD40L, anti-IgM, IL-21, CpG, IFN-α, IL-6 or BAFF induces miR-155 and decreases PU.1 expression. Finally, inhibition of endogenous miR-155 in B cells of RA patients restores PU.1 and reduces production of antibodies. Our data suggest that miR-155 is an important regulator of B-cell activation in RA.",

keywords = "Arthritis, B cells, Comorbidities, Diagnosis algorithm, Psoriasis, Psoriatic arthritis, Rheumatology, miRNA, Arthritis, B cells, Comorbidities, Diagnosis algorithm, Psoriasis, Psoriatic arthritis, Rheumatology, miRNA",

author = "Stefano Alivernini and Mariola Kurowska-Stolarska and Barbara Tolusso and Roberta Benvenuto and Aziza Elmesmari and Silvia Canestri and Luca Petricca and Antonella Mangoni and Fedele, {Anna Laura} and {Di Mario}, Clara and Gigante, {Maria Rita} and Elisa Gremese and Mcinnes, {Iain B.} and Gianfranco Ferraccioli",

year = "2016",

doi = "10.1038/ncomms12970",

language = "English",

volume = "7",

pages = "12970--N/A",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

}

TY - JOUR

T1 - MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis

AU - Alivernini, Stefano

AU - Kurowska-Stolarska, Mariola

AU - Tolusso, Barbara

AU - Benvenuto, Roberta

AU - Elmesmari, Aziza

AU - Canestri, Silvia

AU - Petricca, Luca

AU - Mangoni, Antonella

AU - Fedele, Anna Laura

AU - Di Mario, Clara

AU - Gigante, Maria Rita

AU - Gremese, Elisa

AU - Mcinnes, Iain B.

AU - Ferraccioli, Gianfranco

PY - 2016

Y1 - 2016

N2 - MicroRNA-155 (miR-155) is an important regulator of B cells in mice. B cells have a critical role in the pathogenesis of rheumatoid arthritis (RA). Here we show that miR-155 is highly expressed in peripheral blood B cells from RA patients compared with healthy individuals, particularly in the IgD - CD27 - memory B-cell population in ACPA + RA. MiR-155 is highly expressed in RA B cells from patients with synovial tissue containing ectopic germinal centres compared with diffuse synovial tissue. MiR-155 expression is associated reciprocally with lower expression of PU.1 at B-cell level in the synovial compartment. Stimulation of healthy donor B cells with CD40L, anti-IgM, IL-21, CpG, IFN-α, IL-6 or BAFF induces miR-155 and decreases PU.1 expression. Finally, inhibition of endogenous miR-155 in B cells of RA patients restores PU.1 and reduces production of antibodies. Our data suggest that miR-155 is an important regulator of B-cell activation in RA.

AB - MicroRNA-155 (miR-155) is an important regulator of B cells in mice. B cells have a critical role in the pathogenesis of rheumatoid arthritis (RA). Here we show that miR-155 is highly expressed in peripheral blood B cells from RA patients compared with healthy individuals, particularly in the IgD - CD27 - memory B-cell population in ACPA + RA. MiR-155 is highly expressed in RA B cells from patients with synovial tissue containing ectopic germinal centres compared with diffuse synovial tissue. MiR-155 expression is associated reciprocally with lower expression of PU.1 at B-cell level in the synovial compartment. Stimulation of healthy donor B cells with CD40L, anti-IgM, IL-21, CpG, IFN-α, IL-6 or BAFF induces miR-155 and decreases PU.1 expression. Finally, inhibition of endogenous miR-155 in B cells of RA patients restores PU.1 and reduces production of antibodies. Our data suggest that miR-155 is an important regulator of B-cell activation in RA.

KW - Arthritis

KW - B cells

KW - Comorbidities

KW - Diagnosis algorithm

KW - Psoriasis

KW - Psoriatic arthritis

KW - Rheumatology

KW - miRNA

KW - Arthritis

KW - B cells

KW - Comorbidities

KW - Diagnosis algorithm

KW - Psoriasis

KW - Psoriatic arthritis

KW - Rheumatology

KW - miRNA

UR - http://hdl.handle.net/10807/94038

UR - http://www.nature.com/ncomms/index.html

U2 - 10.1038/ncomms12970

DO - 10.1038/ncomms12970

M3 - Article

SN - 2041-1723

VL - 7

SP - 12970-N/A

JO - Nature Communications

JF - Nature Communications

ER -

MicroRNA-155 influences B-cell function through PU.1 in rheumatoid arthritis

Abstract

Keywords

Accesso al documento

Altri file e collegamenti

Fingerprint

Cita questo