TY - JOUR
T1 - Microbial tracking of multidrug-resistant Klebsiella pneumoniae isolates in a pediatric hospital setting
AU - Bernaschi, P.
AU - Del Chierico, F.
AU - Petrucca, A.
AU - Argentieri, A.
AU - Ciofi Degli Atti, M.
AU - Ciliento, G.
AU - Carletti, M.
AU - Muraca, M.
AU - Locatelli, Franco
AU - Putignani, Lorenza
PY - 2013
Y1 - 2013
N2 - We investigated the clonal relatedness of seven multi-drug-resistant (MDR) Klebsiella pneumoniae isolates, as well as three susceptible K pneumoniae isolates collected during hospital outbreaks and outbreak-related microbiological surveillance, respectively. The relatedness among K pneumoniae isolates was assessed by pulsed field gel electrophoresis (PFGE) and automated repetitive-sequence-based PCR (rep-PCR) genotyping and the results were compared to a proteomic phenotyping performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All typing methods agreed on the generation of three different clusters of K. pneumoniae isogenetic/related MDR strains. After strengthening hospital infection control measures, no other spreading events involving MDR-K pneumoniae were reported until the end of the observation period. This preliminary investigation suggests that, in a hierarchical approach to bacterial typing, MALDI-TOF MS proteome profiling might offer a fast and valuable preliminary screening tool able to support microbiologists during nosocomial outbreak surveys.
AB - We investigated the clonal relatedness of seven multi-drug-resistant (MDR) Klebsiella pneumoniae isolates, as well as three susceptible K pneumoniae isolates collected during hospital outbreaks and outbreak-related microbiological surveillance, respectively. The relatedness among K pneumoniae isolates was assessed by pulsed field gel electrophoresis (PFGE) and automated repetitive-sequence-based PCR (rep-PCR) genotyping and the results were compared to a proteomic phenotyping performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). All typing methods agreed on the generation of three different clusters of K. pneumoniae isogenetic/related MDR strains. After strengthening hospital infection control measures, no other spreading events involving MDR-K pneumoniae were reported until the end of the observation period. This preliminary investigation suggests that, in a hierarchical approach to bacterial typing, MALDI-TOF MS proteome profiling might offer a fast and valuable preliminary screening tool able to support microbiologists during nosocomial outbreak surveys.
KW - microbial typing
KW - MALDI-TOF MS proteome profiling
KW - Klebsiella pneumoniae multi-drug-resistant (MDR) strains
KW - microbial typing
KW - MALDI-TOF MS proteome profiling
KW - Klebsiella pneumoniae multi-drug-resistant (MDR) strains
UR - http://hdl.handle.net/10807/244376
U2 - 10.1177/039463201302600219
DO - 10.1177/039463201302600219
M3 - Article
SN - 0394-6320
VL - 26
SP - 463
EP - 472
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
ER -