TY - JOUR
T1 - Microbacterium paulum sp. nov., isolated from microfiltered milk
AU - Bellassi, Paolo
AU - Fontana, Alessandra
AU - Callegari, Maria Luisa
AU - Cappa, Fabrizio
AU - Morelli, Lorenzo
PY - 2021
Y1 - 2021
N2 - A novel Gram-stain-
positive,
strictly aerobic, short rod-shaped
bacterium, designated 2CT, was isolated from freshly packaged
microfiltered milk. This strain was able to grow within the NaCl concentration range of 0–5 % (w/v), temperature range of
8–37 °C (optimally at 30 °C) and at pH 6.0–10.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain
2CT was closely related to species of the genus Microbacterium, with the highest sequence similarity (99.2 %) to Microbacterium
lacticum DSM 20427T as well as Microbacterium flavum DSM 18909T (=YM18-098T).
The phylogenetic tree based on 16S rRNA
genes showed that strain 2CT clustered with M. flavum DSM 18909T. However, the phylogenetic tree based on concatenated 16S
rRNA and four housekeeping genes showed that strain 2CT clustered with M. lacticum DSM 20427T. Furthermore, the phylogenomic
tree showed that strain 2CT clustered with M. lacticum DSM 20427T and M. flavum DSM 18909T. The major respiratory
quinones were MK-10,
MK-11
and MK-12.
The predominant cellular fatty acids were anteiso-C
15 : 0, iso-C
16 : 0 and anteiso-C
17 : 0. The
polar lipid composition of strain 2CT consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids
and two unidentified lipids. The cell-wall
peptidoglycan type was a variant of B1α {Gly} [l-Lys]
d-Glu-
l-
Lys,
with the amino acids
lysine, glycine, alanine and glutamic acid. The whole-cell
sugars consisted of galactose, glucose, ribose and minor amounts
of rhamnose. In addition, strain 2CT showed a glycolyl-type
cell wall. The genomic DNA G+C content was 69.8 mol%, while the
average nucleotide identity (ANI) and digital DNA-DNA
hybridization (dDDH) values with the closely related Microbacterium
species were below the recognized thresholds of 95–96 % ANI and 70 % DDH for species definition. Based on the phenotypic and
genotypic data, strain 2CT (=LMG 32277T=CECT 30329T) is considered to represent a new species, for which the name Microbacterium
paulum sp. nov. is proposed.
AB - A novel Gram-stain-
positive,
strictly aerobic, short rod-shaped
bacterium, designated 2CT, was isolated from freshly packaged
microfiltered milk. This strain was able to grow within the NaCl concentration range of 0–5 % (w/v), temperature range of
8–37 °C (optimally at 30 °C) and at pH 6.0–10.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain
2CT was closely related to species of the genus Microbacterium, with the highest sequence similarity (99.2 %) to Microbacterium
lacticum DSM 20427T as well as Microbacterium flavum DSM 18909T (=YM18-098T).
The phylogenetic tree based on 16S rRNA
genes showed that strain 2CT clustered with M. flavum DSM 18909T. However, the phylogenetic tree based on concatenated 16S
rRNA and four housekeeping genes showed that strain 2CT clustered with M. lacticum DSM 20427T. Furthermore, the phylogenomic
tree showed that strain 2CT clustered with M. lacticum DSM 20427T and M. flavum DSM 18909T. The major respiratory
quinones were MK-10,
MK-11
and MK-12.
The predominant cellular fatty acids were anteiso-C
15 : 0, iso-C
16 : 0 and anteiso-C
17 : 0. The
polar lipid composition of strain 2CT consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids
and two unidentified lipids. The cell-wall
peptidoglycan type was a variant of B1α {Gly} [l-Lys]
d-Glu-
l-
Lys,
with the amino acids
lysine, glycine, alanine and glutamic acid. The whole-cell
sugars consisted of galactose, glucose, ribose and minor amounts
of rhamnose. In addition, strain 2CT showed a glycolyl-type
cell wall. The genomic DNA G+C content was 69.8 mol%, while the
average nucleotide identity (ANI) and digital DNA-DNA
hybridization (dDDH) values with the closely related Microbacterium
species were below the recognized thresholds of 95–96 % ANI and 70 % DDH for species definition. Based on the phenotypic and
genotypic data, strain 2CT (=LMG 32277T=CECT 30329T) is considered to represent a new species, for which the name Microbacterium
paulum sp. nov. is proposed.
KW - Microbacteriaceae
KW - Microbacterium paulum
KW - microfiltered milk
KW - Microbacteriaceae
KW - Microbacterium paulum
KW - microfiltered milk
UR - http://hdl.handle.net/10807/189526
U2 - 10.1099/ijsem.0.005119
DO - 10.1099/ijsem.0.005119
M3 - Article
SN - 1466-5026
SP - 1
EP - 10
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
ER -