Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders

Giovanni Delogu; Teresa Chiacchio; Valentina Vanini; Ornella Butera; Gilda Cuzzi; Alessandra Bua; Paola Molicotti; Stefania Zanetti; Francesco Nicola Lauria; Susanna Grisetti; Nicola Magnavita; Giovanni Fadda; Enrico Girardi; Delia Goletti

doi:10.1371/journal.pone.0018315

Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders

Giovanni Delogu
, Teresa Chiacchio
, Valentina Vanini
, Ornella Butera
, Gilda Cuzzi
, Alessandra Bua
, Paola Molicotti
, Stefania Zanetti
, Francesco Nicola Lauria
, Susanna Grisetti
, Nicola Magnavita
, Giovanni Fadda
, Enrico Girardi
, Delia Goletti

IRCCS Istituto per le Malattie Infettive Lazzaro Spallanzani - Roma
University of Sassari

Risultato della ricerca: Contributo in rivista › Articolo

56 Citazioni (Scopus)

Abstract

A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.

Lingua originale	Inglese
pagine (da-a)	e18315-e18315
Rivista	PLoS One
Volume	6
DOI	https://doi.org/10.1371/journal.pone.0018315
Stato di pubblicazione	Pubblicato - 2011

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Keywords

Adult
Antigens, Bacterial
CD4-Positive T-Lymphocytes
Demography
Female
Humans
Immunologic Memory
Interferon-gamma
Lectins
Male
Methylation
Middle Aged
Mycobacterium smegmatis
Mycobacterium tuberculosis
ROC Curve
Reagent Kits, Diagnostic
Recombinant Proteins
Time Factors
Tuberculosis

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10.1371/journal.pone.0018315

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Delogu, G., Chiacchio, T., Vanini, V., Butera, O., Cuzzi, G., Bua, A., Molicotti, P., Zanetti, S., Lauria, F. N., Grisetti, S., Magnavita, N., Fadda, G., Girardi, E., & Goletti, D. (2011). Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders. PLoS One, 6, e18315-e18315. https://doi.org/10.1371/journal.pone.0018315

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title = "Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders",

abstract = "A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.",

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author = "Giovanni Delogu and Teresa Chiacchio and Valentina Vanini and Ornella Butera and Gilda Cuzzi and Alessandra Bua and Paola Molicotti and Stefania Zanetti and Lauria, \{Francesco Nicola\} and Susanna Grisetti and Nicola Magnavita and Giovanni Fadda and Enrico Girardi and Delia Goletti",

year = "2011",

doi = "10.1371/journal.pone.0018315",

language = "English",

volume = "6",

pages = "e18315--e18315",

journal = "PLoS One",

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publisher = "Public Library of Science",

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Delogu, G, Chiacchio, T, Vanini, V, Butera, O, Cuzzi, G, Bua, A, Molicotti, P, Zanetti, S, Lauria, FN, Grisetti, S, Magnavita, N, Fadda, G, Girardi, E & Goletti, D 2011, 'Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders', PLoS One, vol. 6, pagg. e18315-e18315. https://doi.org/10.1371/journal.pone.0018315

TY - JOUR

T1 - Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders

AU - Delogu, Giovanni

AU - Chiacchio, Teresa

AU - Vanini, Valentina

AU - Butera, Ornella

AU - Cuzzi, Gilda

AU - Bua, Alessandra

AU - Molicotti, Paola

AU - Zanetti, Stefania

AU - Lauria, Francesco Nicola

AU - Grisetti, Susanna

AU - Magnavita, Nicola

AU - Fadda, Giovanni

AU - Girardi, Enrico

AU - Goletti, Delia

PY - 2011

Y1 - 2011

N2 - A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.

AB - A challenge in tuberculosis (TB) research is to develop a new immunological test that can help distinguish, among subjects responsive to QuantiFERON TB Gold In tube (QFT-IT), those who are able to control Mtb replication (remote LTBI, recent infection and past TB) from those who cannot (active TB disease). IFN-γ response to the Heparin-binding-hemagglutinin (HBHA) of Mtb has been associated with LTBI, but the cumbersome procedures of purifying the methylated and immunological active form of the protein from Mtb or M. bovis Bacillus Calmette et Guerin (BCG) have prevented its implementation in a diagnostic test. Therefore, the aim of the present study was to evaluate the IFN-γ response to methylated HBHA of Mtb produced in M. smegmatis (rHBHAms) in individuals at different stages of TB who scored positive to QFT-IT.

KW - Adult

KW - Antigens, Bacterial

KW - CD4-Positive T-Lymphocytes

KW - Demography

KW - Female

KW - Humans

KW - Immunologic Memory

KW - Interferon-gamma

KW - Lectins

KW - Male

KW - Methylation

KW - Middle Aged

KW - Mycobacterium smegmatis

KW - Mycobacterium tuberculosis

KW - ROC Curve

KW - Reagent Kits, Diagnostic

KW - Recombinant Proteins

KW - Time Factors

KW - Tuberculosis

KW - Adult

KW - Antigens, Bacterial

KW - CD4-Positive T-Lymphocytes

KW - Demography

KW - Female

KW - Humans

KW - Immunologic Memory

KW - Interferon-gamma

KW - Lectins

KW - Male

KW - Methylation

KW - Middle Aged

KW - Mycobacterium smegmatis

KW - Mycobacterium tuberculosis

KW - ROC Curve

KW - Reagent Kits, Diagnostic

KW - Recombinant Proteins

KW - Time Factors

KW - Tuberculosis

UR - http://hdl.handle.net/10807/31262

U2 - 10.1371/journal.pone.0018315

DO - 10.1371/journal.pone.0018315

M3 - Article

SN - 1932-6203

VL - 6

SP - e18315-e18315

JO - PLoS One

JF - PLoS One

ER -

Methylated HBHA produced in M. smegmatis discriminates between active and non-active tuberculosis disease among RD1-responders

Abstract

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Keywords

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