Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods

A. Espinel-Ingroff; J. Turnidge; A. Alastruey-Izquierdo; F. Botterel; E. Canton; C. Castro; Y. -C. Chen; Y. Chen; E. Chryssanthou; E. Dannaoui; G. Garcia-Effron; G. M. Gonzalez; N. P. Govender; J. Guinea; S. Kidd; M. Lackner; C. Lass-Flörl; M. J. Linares-Sicilia; L. López-Soria; R. Magobo; T. Pelaez; G. Quindós; M. A. Rodriguez-Iglesia; M. A. Ruiz; F. Sánchez-Reus; Maurizio Sanguinetti; R. Shields; P. Szweda; A. Tortorano; N. L. Wengenack; S. Bramati; C. Cavanna; C. DeLuca; M. Gelmi; A. Grancini; Gianmarco Lombardi; J. Meletiadis; C. E. Negri; M. Passera; J. Peman; A. Prigitano; E. Sala; M. Tejada

doi:10.1128/AAC.01651-18

Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods

A. Espinel-Ingroff^*
, J. Turnidge
, A. Alastruey-Izquierdo
, F. Botterel
, E. Canton
, C. Castro
, Y. -C. Chen
, Y. Chen
, E. Chryssanthou
, E. Dannaoui
, G. Garcia-Effron
, G. M. Gonzalez
, N. P. Govender
, J. Guinea
, S. Kidd
, M. Lackner
, C. Lass-Flörl
, M. J. Linares-Sicilia
, L. López-Soria
, R. Magobo

T. Pelaez, G. Quindós, M. A. Rodriguez-Iglesia, M. A. Ruiz, F. Sánchez-Reus, Maurizio Sanguinetti, R. Shields, P. Szweda, A. Tortorano, N. L. Wengenack, S. Bramati, C. Cavanna, C. DeLuca, M. Gelmi, A. Grancini, Gianmarco Lombardi, J. Meletiadis, C. E. Negri, M. Passera, J. Peman, A. Prigitano, E. Sala, M. Tejada

^*Autore corrispondente per questo lavoro

Virginia Commonwealth University
University of Adelaide
Centro Nacional de Microbiologia
Unité de Parasitologie
Instituto Investigacion Sanitaria la Fe
Hospital Universitario de Valme
Public Health Ontario
Karolinska Institutet
Université de Paris
Universidad Nacional del Litoral
Universidad Autonoma de Nuevo Leon
University of the Witwatersrand
Hospital General Universitario Gregorio Marañon
National Mycology Reference Centre
Innsbruck Medical University
University of Córdoba
Hospital de Cruces
Hospital Universitario Central de Asturias
University of the Basque Country
University of Cádiz
Instituto de Investigación Sanitaria Aragón (IISA)
Hematology, Hospital Santa Creu i Sant Pau
University of Pittsburgh
Gdańsk University of Technology
University of Milan
Mayo Clinic Rochester, MN
Azienda Ospedaliera San Gerardo Monza
IRCCS Fondazione Policlinico San Matteo - Pavia
IRCCS Istituto Clinico Humanitas - Rozzano (Milano)
Spedali Civili Di Brescia
IRCCS Fondazione Ca'Granda – Ospedale Maggiore Policlinico - Milano
National and Kapodistrian University of Athens
Universidade Federal de São Paulo
ASST Papa Giovanni XXIII
Hospital Universitario La Fe
ASST Lariana
IRCCS Policlinico San Donato

Risultato della ricerca: Contributo in rivista › Articolo

32 Citazioni (Scopus)

Abstract

Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. Niger, and 302 A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 g/ml for C. albicans and the Etest itraconazole ECV of 2 g/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.

Lingua originale	Inglese
pagine (da-a)	N/A-N/A
Rivista	Antimicrobial Agents and Chemotherapy
Volume	63
Numero di pubblicazione	1
DOI	https://doi.org/10.1128/AAC.01651-18
Stato di pubblicazione	Pubblicato - 2019

OSS delle Nazioni Unite

Questo processo contribuisce al raggiungimento dei seguenti obiettivi di sviluppo sostenibile

SDG 3 Salute e benessere

All Science Journal Classification (ASJC) codes

Farmacologia
Farmacologia (medica)
Malattie Infettive

Keywords

Antifungal resistance
Aspergillus spp
Candida albicans
Candida glabrata
Etest MICs for fungal mutants
Etest method ECVs
Infectious Diseases
Pharmacology
Pharmacology (medical)
SYO MICs for fungal mutants
SYO method ECVs
Triazole ECVs

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10.1128/AAC.01651-18

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Espinel-Ingroff, A., Turnidge, J., Alastruey-Izquierdo, A., Botterel, F., Canton, E., Castro, C., Chen, Y. .-C., Chen, Y., Chryssanthou, E., Dannaoui, E., Garcia-Effron, G., Gonzalez, G. M., Govender, N. P., Guinea, J., Kidd, S., Lackner, M., Lass-Flörl, C., Linares-Sicilia, M. J., López-Soria, L., ... Tejada, M. (2019). Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods. Antimicrobial Agents and Chemotherapy, 63(1), N/A-N/A. https://doi.org/10.1128/AAC.01651-18

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title = "Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods",

abstract = "Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. Niger, and 302 A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 g/ml for C. albicans and the Etest itraconazole ECV of 2 g/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.",

keywords = "Antifungal resistance, Aspergillus spp, Candida albicans, Candida glabrata, Etest MICs for fungal mutants, Etest method ECVs, Infectious Diseases, Pharmacology, Pharmacology (medical), SYO MICs for fungal mutants, SYO method ECVs, Triazole ECVs, Antifungal resistance, Aspergillus spp, Candida albicans, Candida glabrata, Etest MICs for fungal mutants, Etest method ECVs, Infectious Diseases, Pharmacology, Pharmacology (medical), SYO MICs for fungal mutants, SYO method ECVs, Triazole ECVs",

author = "A. Espinel-Ingroff and J. Turnidge and A. Alastruey-Izquierdo and F. Botterel and E. Canton and C. Castro and Chen, \{Y. -C.\} and Y. Chen and E. Chryssanthou and E. Dannaoui and G. Garcia-Effron and Gonzalez, \{G. M.\} and Govender, \{N. P.\} and J. Guinea and S. Kidd and M. Lackner and C. Lass-Fl{\"o}rl and Linares-Sicilia, \{M. J.\} and L. L{\'o}pez-Soria and R. Magobo and T. Pelaez and G. Quind{\'o}s and Rodriguez-Iglesia, \{M. A.\} and Ruiz, \{M. A.\} and F. S{\'a}nchez-Reus and Maurizio Sanguinetti and R. Shields and P. Szweda and A. Tortorano and Wengenack, \{N. L.\} and S. Bramati and C. Cavanna and C. DeLuca and M. Gelmi and A. Grancini and Gianmarco Lombardi and J. Meletiadis and Negri, \{C. E.\} and M. Passera and J. Peman and A. Prigitano and E. Sala and M. Tejada",

year = "2019",

doi = "10.1128/AAC.01651-18",

language = "English",

volume = "63",

pages = "N/A--N/A",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "1",

}

Espinel-Ingroff, A, Turnidge, J, Alastruey-Izquierdo, A, Botterel, F, Canton, E, Castro, C, Chen, Y-C, Chen, Y, Chryssanthou, E, Dannaoui, E, Garcia-Effron, G, Gonzalez, GM, Govender, NP, Guinea, J, Kidd, S, Lackner, M, Lass-Flörl, C, Linares-Sicilia, MJ, López-Soria, L, Magobo, R, Pelaez, T, Quindós, G, Rodriguez-Iglesia, MA, Ruiz, MA, Sánchez-Reus, F, Sanguinetti, M, Shields, R, Szweda, P, Tortorano, A, Wengenack, NL, Bramati, S, Cavanna, C, DeLuca, C, Gelmi, M, Grancini, A, Lombardi, G, Meletiadis, J, Negri, CE, Passera, M, Peman, J, Prigitano, A, Sala, E & Tejada, M 2019, 'Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods', Antimicrobial Agents and Chemotherapy, vol. 63, n. 1, pagg. N/A-N/A. https://doi.org/10.1128/AAC.01651-18

Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods. / Espinel-Ingroff, A.; Turnidge, J.; Alastruey-Izquierdo, A. et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 63, N. 1, 2019, pag. N/A-N/A.

Risultato della ricerca: Contributo in rivista › Articolo

TY - JOUR

T1 - Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods

AU - Espinel-Ingroff, A.

AU - Turnidge, J.

AU - Alastruey-Izquierdo, A.

AU - Botterel, F.

AU - Canton, E.

AU - Castro, C.

AU - Chen, Y. -C.

AU - Chen, Y.

AU - Chryssanthou, E.

AU - Dannaoui, E.

AU - Garcia-Effron, G.

AU - Gonzalez, G. M.

AU - Govender, N. P.

AU - Guinea, J.

AU - Kidd, S.

AU - Lackner, M.

AU - Lass-Flörl, C.

AU - Linares-Sicilia, M. J.

AU - López-Soria, L.

AU - Magobo, R.

AU - Pelaez, T.

AU - Quindós, G.

AU - Rodriguez-Iglesia, M. A.

AU - Ruiz, M. A.

AU - Sánchez-Reus, F.

AU - Sanguinetti, Maurizio

AU - Shields, R.

AU - Szweda, P.

AU - Tortorano, A.

AU - Wengenack, N. L.

AU - Bramati, S.

AU - Cavanna, C.

AU - DeLuca, C.

AU - Gelmi, M.

AU - Grancini, A.

AU - Lombardi, Gianmarco

AU - Meletiadis, J.

AU - Negri, C. E.

AU - Passera, M.

AU - Peman, J.

AU - Prigitano, A.

AU - Sala, E.

AU - Tejada, M.

PY - 2019

Y1 - 2019

N2 - Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. Niger, and 302 A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 g/ml for C. albicans and the Etest itraconazole ECV of 2 g/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.

AB - Although the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex, 157 C. guilliermondii (Meyerozyma guilliermondii), 676 C. krusei (Pichia kudriavzevii), 298 C. lusitaniae (Clavispora lusitaniae), 911 C. parapsilosis sensu stricto, 3,691 C. parapsilosis species complex, 36 C. metapsilosis, 110 C. orthopsilosis, 1,854 C. tropicalis, 244 Saccharomyces cerevisiae, 1,409 Aspergillus fumigatus, 389 A. flavus, 130 A. nidulans, 233 A. Niger, and 302 A. terreus complex isolates. SYO/Etest MICs for 282 confirmed non-wild-type (non-WT) isolates were included: ERG11 (C. albicans), ERG11 and MRR1 (C. parapsilosis), cyp51A (A. fumigatus), and CDR2 and CDR1 overexpression (C. albicans and C. glabrata, respectively). Interlaboratory modal agreement was superior by SYO for yeast species and by the Etest for Aspergillus spp. Distributions fulfilling CLSI criteria for epidemiological cutoff value (ECV) definition were pooled, and we proposed SYO ECVs for S. cerevisiae and 9 yeast and 3 Aspergillus species and Etest ECVs for 5 yeast and 4 Aspergillus species. The posaconazole SYO ECV of 0.06 g/ml for C. albicans and the Etest itraconazole ECV of 2 g/ml for A. fumigatus were the best predictors of non-WT isolates. These findings support the need for method-dependent ECVs, as, overall, the SYO appears to perform better for susceptibility testing of yeast species and the Etest appears to perform better for susceptibility testing of Aspergillus spp. Further evaluations should be conducted with more Candida mutants.

KW - Antifungal resistance

KW - Aspergillus spp

KW - Candida albicans

KW - Candida glabrata

KW - Etest MICs for fungal mutants

KW - Etest method ECVs

KW - Infectious Diseases

KW - Pharmacology

KW - Pharmacology (medical)

KW - SYO MICs for fungal mutants

KW - SYO method ECVs

KW - Triazole ECVs

KW - Antifungal resistance

KW - Aspergillus spp

KW - Candida albicans

KW - Candida glabrata

KW - Etest MICs for fungal mutants

KW - Etest method ECVs

KW - Infectious Diseases

KW - Pharmacology

KW - Pharmacology (medical)

KW - SYO MICs for fungal mutants

KW - SYO method ECVs

KW - Triazole ECVs

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U2 - 10.1128/AAC.01651-18

DO - 10.1128/AAC.01651-18

M3 - Article

SN - 0066-4804

VL - 63

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 1

ER -

Espinel-Ingroff A, Turnidge J, Alastruey-Izquierdo A, Botterel F, Canton E, Castro C et al. Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods. Antimicrobial Agents and Chemotherapy. 2019;63(1):N/A-N/A. doi: 10.1128/AAC.01651-18

Method-dependent epidemiological cutoff values for detection of triazole resistance in Candida and Aspergillus species for the Sensititre Yeastone colorimetric broth and etest agar diffusion methods

Abstract

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All Science Journal Classification (ASJC) codes

Keywords

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