Metaproteomic investigation to assess gut microbiota shaping in newborn mice: A combined taxonomic, functional and quantitative approach

Stefano Levi Mortera, Alessio Soggiu, Pamela Vernocchi, Federica Del Chierico, Cristian Piras, Rita Carsetti, Valeria Marzano, Domenico Britti, Andrea Urbani, Paola Roncada, Lorenza Putignani

Risultato della ricerca: Contributo in rivistaArticolo in rivista

3 Citazioni (Scopus)


Breastfeeding is nowadays known to be one of the most critical factors contributing to the development of an efficient immune system. In the last decade, a consistent number of pieces of evidence demonstrated the relationship between a healthy organism and its gut microbiota. However, this link is still not fully understood and requires further investigation. We recently adopted a murine model to describe the impact of either maternal milk or parental genetic background, on the composition of the gut microbial population in the first weeks of life. A metaproteomic approach to such complex environments is a big challenge that requires a strong effort in both data production and analysis, including the set-up of dedicated multitasking bioinformatics pipelines. Herein we present an LC-MS/MS based investigation to monitor mouse gut microbiota in the early life, aiming at characterizing its functions and metabolic activities together with a taxonomic description in terms of operational taxonomic units. We provided a quantitative evaluation of bacterial metaproteins, taking into account differential expression results in relation to the functional and taxonomic classification, particularly with proteins from orthologues groups. This allowed the reduction of the bias arising from the presence of a high number of shared peptides, and proteins, among different bacterial species. We also focused on host mucosal proteome and its modulation, according to different microbiota composition.
Lingua originaleEnglish
pagine (da-a)84-92
Numero di pagine9
RivistaJournal of Proteomics
Stato di pubblicazionePubblicato - 2019


  • Metaproteomics
  • Mouse gut microbiota shaping


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