Metabolism of straight saturated medium chain length (C9 to C12) dicarboxylic acids

Geltrude Mingrone, S Passi, M Nazzaro Porro, M Picardo, P. Fasella

Risultato della ricerca: Contributo in rivistaArticolo in rivista

41 Citazioni (Scopus)

Abstract

A method utilizing thin-layer chromatography, high performance liquid chromatography, and mass spectrometry was developed for the quantification of C9, C10, C11, and C12 dicarboxylic acids in serum, urine, and feces of human volunteers and rats after oral administration of the acids. The method allowed good resolution and measurement of the dicarboxylic acids at nanogram levels. In humans, excretion was independent of the dosage; about 60% of C9, 17% of C10, 5% of C11, and 1% of C12 were excreted in the urine during the first 12 hours after administration. The concentration of the acids in serum peaked between 2 and 3 hours. Excretion was also independent of dosage in rats. About 2.5% of C, 2.1% of C10, 1.8% of C11, and 1.6% of C12 were excreted in the urine over a period of 5 days. The serum concentration and the urinary excretion of the diacids reached a maximum at the second day after the oral dose. In both humans and rats, the dicarboxylic acids found in serum and urine were 2, 4, or 6 carbon atoms shorter than the corresponding administered diacid. This indicates that there was beta-oxidation of the ingested diacids to some extent. The administration of [1,9-14C]azeliac acid and of [10,11-3H]dodecandioic acid confirmed the occurrence of beta-oxidation, and led to elucidation of the fate of the ingested diacids that were not excreted as such in the urine.
Lingua originaleEnglish
pagine (da-a)1140-1147
Numero di pagine8
RivistaJournal of Lipid Research
Volume24
Stato di pubblicazionePubblicato - 1983

Keywords

  • Administration, Oral
  • Adult
  • Animals
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Dicarboxylic Acids
  • Female
  • Humans
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Rats
  • Rats, Inbred Strains
  • Species Specificity

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