Metabolic, enzymatic and gene involvement in cerebral glucose dysmetabolism after traumatic brain injury

Barbara Tavazzi, Angela Maria Amorini, Giacomo Lazzarino, Valentina Di Pietro, Giuseppe Lazzarino, Stefano Signoretti, Antonio Belli

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

27 Citazioni (Scopus)

Abstract

In this study, the metabolic, enzymatic and gene changes causing cerebral glucose dysmetabolism following graded diffuse traumatic brain injury (TBI) were evaluated. TBI was induced in rats by dropping 450g from 1 (mild TBI; mTBI) or 2m height (severe TBI; sTBI). After 6, 12, 24, 48, and 120h gene expressions and enzymatic activities of glycolysis and pentose phosphate pathway (PPP) enzymes, and levels of lactate, ATP, ADP, ATP/ADP (indexing mitochondrial phosphorylating capacity), NADP(+), NADPH and GSH were determined in whole brain extracts (n=9 rats at each time for both TBI levels). Sham-operated animals (n=9) were used as controls. Results demonstrated that mTBI caused a late increase (48-120h post injury) of glycolytic gene expression and enzymatic activities, concomitantly with mitochondrial functional recovery (ATP and ATP/ADP normalization). No changes in lactate and PPP genes and enzymes, were accompanied by transient decrease in GSH, NADP(+), NADPH and NADPH/NADP(+). Animals following sTBI showed early increase (6-24h post injury) of glycolytic gene expression and enzymatic activities, occurring during mitochondrial malfunctioning (50% decrease in ATP and ATP/ADP). Higher lactate and lower GSH, NADP(+), NADPH, NADPH/NADP(+) than controls were recorded at anytime post injury (p<0.01). Both TBI levels caused metabolic and gene changes affecting glucose metabolism. Following mTBI, increased glucose flux through glycolysis is coupled to mitochondrial glucose oxidation. "True" hyperglycolysis occurs only after sTBI, where metabolic changes, caused by depressed mitochondrial phosphorylating capacity, act on genes causing net glycolytic flux increase uncoupled from mitochondrial glucose oxidation.
Lingua originaleEnglish
pagine (da-a)679-687-687
Numero di pagine8
RivistaBIOCHIMICA ET BIOPHYSICA ACTA
Volume1862
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Energy metabolism
  • Glucose dysmetabolism
  • Glycolysis
  • Mitochondrial dysfunction
  • Pentose phosphate pathway
  • Traumatic brain injury

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