TY - JOUR
T1 - METABOLIC ALTERATIONS INDUCED BY METHACRILIC MONOMERS IN HUMAN PULPAR FIBROBLASTS
AU - Calla', Cinzia Anna Maria
AU - Lupi, A.
AU - Gozzo, M. L.
AU - Cicillini, L.
AU - Giardina, B.
AU - Nocca, Giuseppina
PY - 2012
Y1 - 2012
N2 - Aim: Composite resins were introduced in the 1960s
for the restoring of anterior teeth in substitution
of the amalgam that presented both aesthetical
and biocompatibility problems. However, since the
polymerization of methacrylates is never complete, the
need to appraise the biocompatibility of composite resins
became evident. The incomplete conversion causes in
fact the release of monomers that may implement adverse
effects in the organism, i.e. allergic reaction, systemic
toxicity, cytotoxicity. Given that very little information
has been so far delivered on the effects of methacrylic
monomers on cell metabolism, the aim of this work was
the evaluation of the biochemical interactions between
methacrylates and human fibroblasts.
Methods: The effects of triethylenglycol-dimethacrylate
(TEGDMA), 2-hydroxyhethyl methacrylate (HEMA), and
1,4-butanediol dimethacrylate (BDDMA) on 1) cellular
energetic metabolism (oxygen consumption rate, glucose
consumption, G6PDH, lactate production) 2) cellular
redox status (GSH concentration, the activity of the
enzymes regulating glutathione metabolism and REDOX
status of cells) were analysed according to Nocca et al.
Data are expressed as the mean ± statistical error of
the mean (SEM). The means were compared by analysis
of variance (ANOVA) followed, when appropriate, by a
multiple comparison of means by Student-Newman-Keuls
test: p < 0.05 was considered significant.
Results: The results showed that TEGDMA, HEMA and
BDDMA induce a statically significant decrease of oxygen
consumption, an enhancement of glucose consumption,
lactate production and also GSH depletion.
Conclusions: The alterations in energy metabolism,
REDOX status and glutathione balance – produced in the
cellular metabolism by HEMA, TEGDMA and BDDMA –
can be considered among the mechanisms inducing the
clinical and sub-clinical adverse effects that have been
occasionally reported during the use of dental resins. In
our opinion, such investigations can therefore be helpful
to test the behavior of materials especially designed to be
applied inside the human body.
Nocca G, De Palma F, Minucci A, et al. Alterations of
energy metabolism and glutathione levels of HL-60 cells
induced by methacrylates present in composite resins. J
Dent 2007;35:187-94.
AB - Aim: Composite resins were introduced in the 1960s
for the restoring of anterior teeth in substitution
of the amalgam that presented both aesthetical
and biocompatibility problems. However, since the
polymerization of methacrylates is never complete, the
need to appraise the biocompatibility of composite resins
became evident. The incomplete conversion causes in
fact the release of monomers that may implement adverse
effects in the organism, i.e. allergic reaction, systemic
toxicity, cytotoxicity. Given that very little information
has been so far delivered on the effects of methacrylic
monomers on cell metabolism, the aim of this work was
the evaluation of the biochemical interactions between
methacrylates and human fibroblasts.
Methods: The effects of triethylenglycol-dimethacrylate
(TEGDMA), 2-hydroxyhethyl methacrylate (HEMA), and
1,4-butanediol dimethacrylate (BDDMA) on 1) cellular
energetic metabolism (oxygen consumption rate, glucose
consumption, G6PDH, lactate production) 2) cellular
redox status (GSH concentration, the activity of the
enzymes regulating glutathione metabolism and REDOX
status of cells) were analysed according to Nocca et al.
Data are expressed as the mean ± statistical error of
the mean (SEM). The means were compared by analysis
of variance (ANOVA) followed, when appropriate, by a
multiple comparison of means by Student-Newman-Keuls
test: p < 0.05 was considered significant.
Results: The results showed that TEGDMA, HEMA and
BDDMA induce a statically significant decrease of oxygen
consumption, an enhancement of glucose consumption,
lactate production and also GSH depletion.
Conclusions: The alterations in energy metabolism,
REDOX status and glutathione balance – produced in the
cellular metabolism by HEMA, TEGDMA and BDDMA –
can be considered among the mechanisms inducing the
clinical and sub-clinical adverse effects that have been
occasionally reported during the use of dental resins. In
our opinion, such investigations can therefore be helpful
to test the behavior of materials especially designed to be
applied inside the human body.
Nocca G, De Palma F, Minucci A, et al. Alterations of
energy metabolism and glutathione levels of HL-60 cells
induced by methacrylates present in composite resins. J
Dent 2007;35:187-94.
KW - methacrylic monomers
KW - pulpar fibroblasts
KW - methacrylic monomers
KW - pulpar fibroblasts
UR - http://hdl.handle.net/10807/61815
M3 - Conference article
SN - 0393-0564
SP - 571
EP - 571
JO - Biochimica Clinica: VOL.36 N.6
JF - Biochimica Clinica: VOL.36 N.6
T2 - 44° Congresso Nazionale Sibioc
Y2 - 5 November 2012 through 8 November 2012
ER -