Membrane bioreactors as hybrid artificial pancreas: experimental evaluation

Celestino Pio Lombardi, Giuseppe Brisinda, Rocco Domenico Alfonso Bellantone, Giovanni Ghirlanda, Franco Ceriati, A Urso, G Catapano, G Careddu, Gb Doglietto, F. Crucitti

Risultato della ricerca: Contributo in rivistaArticolo in rivista

5 Citazioni (Scopus)

Abstract

Results of cultured islet transplantation in the management of insulin-dependent diabetes are still unsatisfactory. The main problem preventing success is the swift and resolute host immune rejection. To obviate this we designed and experimented a model of bioartificial pancreas, made of polymeric hollow fibers, put into the blood circulation as an artero-venous bypass to immunoisolate endocrine tissue from leucocytes and immunoglobulins. We tested four different membrane bioreactors (BR1-4). BR1 and 2 had seven hollow fibers, the others more than 6,000 smaller fibers. In BR4 a connecting tube with a high-permeability membrane was inserted between the islet compartment and the bioreactor outlet to improve the ultrafiltration flow. In vitro, the islets inside the bioreactor perfused with glucose solutions (300 mg%) showed a rapid, high insulin secretory response, related to the glucose stimulation. The use of the outside connection allowed a twofold increase of insulin production.
Lingua originaleEnglish
pagine (da-a)126-130
Numero di pagine5
RivistaInternational Journal of Artificial Organs
Volume15
Stato di pubblicazionePubblicato - 1992

Keywords

  • Animals
  • Cells, Cultured
  • Drug Compounding
  • Insulin
  • Insulin Infusion Systems
  • Islets of Langerhans
  • Islets of Langerhans Transplantation
  • Pancreatectomy
  • Swine

Fingerprint

Entra nei temi di ricerca di 'Membrane bioreactors as hybrid artificial pancreas: experimental evaluation'. Insieme formano una fingerprint unica.

Cita questo