TY - JOUR
T1 - Melamine in eggs, plasma and tissues of hens fed contaminated diets
AU - Gallo, Antonio
AU - Bertuzzi, Terenzio
AU - Battaglia, Marco
AU - Masoero, Francesco
AU - Piva, Gianfranco
AU - Moschini, Maurizio
PY - 2012
Y1 - 2012
N2 - A study was conducted to evaluate the excretion pattern of melamine from feed into eggs, plasma, kidney, liver and muscle of
laying hens. In particular, 90 laying hens were randomly allocated to three dietary treatments and fed diets contaminated with
melamine at a level of 2.5, 25 and 250 mg of melamine/kg of diet for T1, T2 and T3 groups, respectively. The diets were offered in
six replicate boxes (five hens each) for 13 days. Eggs were collected from each group for melamine quantification on days 0, 1, 3,
6, 9 and 13. At the end of the experimental period, one hen per box was randomly selected and slaughtered to collect plasma,
liver, kidney and muscle samples. During the experiment, feeding diets with increasing levels of melamine had no effect ( P.0.05)
on weight gain, feed intake, egg production, egg weight and mortality of laying hens. The melamine in eggs increased from day 1
after melamine ingestion and reached a plateau between days 6 and 13 of melamine ingestion. At steady-state condition, the
melamine egg concentrations increased ( P,0.01) with treatments, being 0.026, 0.352 and 4.631 mg/kg for T1, T2 and T3,
respectively. Similarly, the carryover of melamine from feed to egg increased ( P,0.05) with the levels of melamine in the diets,
varying from 0.50 to 0.70 and 0.84 for T1, T2 and T3, respectively. The melamine was detected in plasma of all tested groups,
increasing ( P,0.01) with levels of melamine in the diets (0.030, 0.266 and 4.102 mg/l in T1, T2 and T3, respectively). Melamine
was not detected in kidney, liver and muscle of hens fed T1. Except for kidney sampled in the T3, no melamine concentration
higher than 2.5 mg/kg, representing the maximum allowable limit set by the US Food and Drug Administration and European
Union for food and feeds, was measured. The melamine resulted higher in plasma and kidneys than in the liver and muscle both
in T2 and T3. The results confirmed the presence of an excretion pattern of melamine from feed to eggs and tissues in laying hen
AB - A study was conducted to evaluate the excretion pattern of melamine from feed into eggs, plasma, kidney, liver and muscle of
laying hens. In particular, 90 laying hens were randomly allocated to three dietary treatments and fed diets contaminated with
melamine at a level of 2.5, 25 and 250 mg of melamine/kg of diet for T1, T2 and T3 groups, respectively. The diets were offered in
six replicate boxes (five hens each) for 13 days. Eggs were collected from each group for melamine quantification on days 0, 1, 3,
6, 9 and 13. At the end of the experimental period, one hen per box was randomly selected and slaughtered to collect plasma,
liver, kidney and muscle samples. During the experiment, feeding diets with increasing levels of melamine had no effect ( P.0.05)
on weight gain, feed intake, egg production, egg weight and mortality of laying hens. The melamine in eggs increased from day 1
after melamine ingestion and reached a plateau between days 6 and 13 of melamine ingestion. At steady-state condition, the
melamine egg concentrations increased ( P,0.01) with treatments, being 0.026, 0.352 and 4.631 mg/kg for T1, T2 and T3,
respectively. Similarly, the carryover of melamine from feed to egg increased ( P,0.05) with the levels of melamine in the diets,
varying from 0.50 to 0.70 and 0.84 for T1, T2 and T3, respectively. The melamine was detected in plasma of all tested groups,
increasing ( P,0.01) with levels of melamine in the diets (0.030, 0.266 and 4.102 mg/l in T1, T2 and T3, respectively). Melamine
was not detected in kidney, liver and muscle of hens fed T1. Except for kidney sampled in the T3, no melamine concentration
higher than 2.5 mg/kg, representing the maximum allowable limit set by the US Food and Drug Administration and European
Union for food and feeds, was measured. The melamine resulted higher in plasma and kidneys than in the liver and muscle both
in T2 and T3. The results confirmed the presence of an excretion pattern of melamine from feed to eggs and tissues in laying hen
KW - EGG
KW - EXCRETION PATTERN
KW - LAYING HEN
KW - MELAMINE
KW - TISSUE
KW - EGG
KW - EXCRETION PATTERN
KW - LAYING HEN
KW - MELAMINE
KW - TISSUE
UR - http://hdl.handle.net/10807/9750
U2 - 10.1017/S1751731111002552
DO - 10.1017/S1751731111002552
M3 - Article
SN - 1751-7311
VL - 6
SP - 1163
EP - 1169
JO - Animal
JF - Animal
ER -