Mechanisms of sevoflurane-induced myocardial preconditioning in isolated human right atria in vitro

Alexandra Yvon, Jean Luc Hanouz, Benoît Haelewyn, Xavier Terrien, Massimo Massetti, Gérard Babatasi, André Khayat, Pierre Ducouret, Henri Bricard, Jean Louis Gérard

Risultato della ricerca: Contributo in rivistaArticolo in rivista

42 Citazioni (Scopus)


BACKGROUND: The authors examined the role of adenosine triphosphate-sensitive potassium channels and adenosine A(1) receptors in sevoflurane-induced preconditioning on isolated human myocardium. METHODS: The authors recorded isometric contraction of human right atrial trabeculae suspended in oxygenated Tyrode's solution (34 degrees C; stimulation frequency, 1 Hz). In all groups, a 30-min hypoxic period was followed by 60 min of reoxygenation. Seven minutes before hypoxia reoxygenation, muscles were exposed to 4 min of hypoxia and 7 min of reoxygenation or 15 min of sevoflurane at concentrations of 1, 2, and 3%. In separate groups, sevoflurane 2% was administered in the presence of 10 microm HMR 1098, a sarcolemmal adenosine triphosphate-sensitive potassium channel antagonist; 800 microm 5-hydroxy-decanoate, a mitochondrial adenosine triphosphate-sensitive potassium channel antagonist; and 100 nm 8-cyclopentyl-1,3-dipropylxanthine, an adenosine A(1) receptor antagonist. Recovery of force at the end of the 60-min reoxygenation period was compared between groups (mean +/- SD). RESULTS: Hypoxic preconditioning (90 +/- 4% of baseline) and sevoflurane 1% (82 +/- 3% of baseline), 2% (92 +/- 5% of baseline), and 3% (85 +/- 7% of baseline) enhanced the recovery of force after 60 min of reoxygenation compared with the control groups (52 +/- 9% of baseline). This effect was abolished in the presence of 5-hydroxy-decanoate (55 +/- 14% of baseline) and 8-cyclopentyl-1,3-dipropylxanthine (58 +/- 16% of baseline) but was attenuated in the presence of HMR 1098 (73 +/- 10% of baseline). CONCLUSIONS: In vitro, sevoflurane preconditions human myocardium against hypoxia through activation of adenosine triphosphate-sensitive potassium channels and stimulation of adenosine A(1) receptors.
Lingua originaleEnglish
pagine (da-a)27-33
Numero di pagine7
Stato di pubblicazionePubblicato - 2003


  • ATP-Binding Cassette Transporters
  • Aged
  • Anesthetics, Inhalation
  • Benzamides
  • Decanoic Acids
  • Heart Atria
  • Humans
  • Hydroxy Acids
  • Hypoxia
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial
  • Isometric Contraction
  • KATP Channels
  • Methyl Ethers
  • Middle Aged
  • Myocardial Contraction
  • Potassium Channel Blockers
  • Potassium Channels
  • Potassium Channels, Inwardly Rectifying
  • Purinergic P1 Receptor Antagonists
  • Receptors, Purinergic P1
  • Reperfusion Injury
  • Sarcolemma
  • Xanthines


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