TY - JOUR
T1 - Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic
Escherichia coli in dog
AU - Piras, Cristian
AU - Soggiu, Alessio
AU - Greco, Viviana
AU - Martino, Piera Anna
AU - Del Chierico, Federica
AU - Putignani, Lorenza
AU - Urbani, Andrea
AU - Nally, Jarlath E.
AU - Bonizzi, Luigi
AU - Roncada, Paola
PY - 2015
Y1 - 2015
N2 - Escherichia coli (E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics.
This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli isolates that represent a good tool to study this pathology.
The isolated E. coli has been induced with enrofloxacin and studied through 2D DICE and shotgun MS.
Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover the mechanism of this clone to counteract the effects of enrofloxacin. In parallel, the dramatic decrease of the synthesis of the outer membrane protein W, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanism of E. coli defense against this antibiotic. All 20 DICE and MS data have been deposited into the ProteomeXchange Consortium with identifier PXD002000 and DOI http://dx.doi.org/10.601/PXD002000.
AB - Escherichia coli (E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics.
This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli isolates that represent a good tool to study this pathology.
The isolated E. coli has been induced with enrofloxacin and studied through 2D DICE and shotgun MS.
Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover the mechanism of this clone to counteract the effects of enrofloxacin. In parallel, the dramatic decrease of the synthesis of the outer membrane protein W, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanism of E. coli defense against this antibiotic. All 20 DICE and MS data have been deposited into the ProteomeXchange Consortium with identifier PXD002000 and DOI http://dx.doi.org/10.601/PXD002000.
KW - URIDINE PHOSPHORYLASE
KW - MASS-SPECTROMETRY
KW - ANTIMICROBIAL RESISTANCE
KW - URIDINE PHOSPHORYLASE
KW - MASS-SPECTROMETRY
KW - ANTIMICROBIAL RESISTANCE
UR - http://hdl.handle.net/10807/234010
U2 - 10.1016/j.jprot.2015.05.040
DO - 10.1016/j.jprot.2015.05.040
M3 - Article
SN - 1874-3919
VL - 127
SP - 365
EP - 376
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -