TY - JOUR
T1 - Measuring the confluence of iPSCs using an automated imaging system
AU - Magliocca, Valentina
AU - Vinci, Maria
AU - Persichini, Tiziana
AU - Locatelli, Franco
AU - Tartaglia, Marco
AU - Compagnucci, Claudia
PY - 2020
Y1 - 2020
N2 - This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.
AB - This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.
KW - PSCs
KW - PSCs
UR - http://hdl.handle.net/10807/228559
U2 - 10.3791/61225
DO - 10.3791/61225
M3 - Article
SN - 1940-087X
VL - 2020
SP - 1
EP - 10
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
ER -