Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

Brunella Posteraro, Maurizio Sanguinetti, Antonietta Vella, Elena De Carolis, Massimo Cogliati, Patrizia Posteraro, Anna Maria Tortorano

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

68 Citazioni (Scopus)

Abstract

We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.
Lingua originaleEnglish
pagine (da-a)2472-2476
Numero di pagine5
RivistaJournal of Clinical Microbiology
Volume50
DOI
Stato di pubblicazionePubblicato - 2012

Keywords

  • Cryptococcus neoformans

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