Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

Brunella Posteraro; Antonietta Vella; Massimo Cogliati; Elena De Carolis; Ada Rita Florio; Patrizia Posteraro; Maurizio Sanguinetti; Anna Maria Tortorano

doi:10.1128/JCM.00737-12

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

Brunella Posteraro, Antonietta Vella, Massimo Cogliati, Elena De Carolis, Ada Rita Florio, Patrizia Posteraro, Maurizio Sanguinetti, Anna Maria Tortorano

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

68 Citazioni (Scopus)

Abstract

We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.

Lingua originale	English
pagine (da-a)	2472-2476
Numero di pagine	5
Rivista	Journal of Clinical Microbiology
Volume	50
DOI	https://doi.org/10.1128/JCM.00737-12
Stato di pubblicazione	Pubblicato - 2012

Keywords

Cryptococcus neoformans

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10.1128/JCM.00737-12

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Posteraro, B., Vella, A., Cogliati, M., De Carolis, E., Florio, A. R., Posteraro, P., Sanguinetti, M., & Tortorano, A. M. (2012). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii. Journal of Clinical Microbiology, 50, 2472-2476. https://doi.org/10.1128/JCM.00737-12

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title = "Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii",

abstract = "We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.",

keywords = "Cryptococcus neoformans, Cryptococcus neoformans",

author = "Brunella Posteraro and Antonietta Vella and Massimo Cogliati and {De Carolis}, Elena and Florio, {Ada Rita} and Patrizia Posteraro and Maurizio Sanguinetti and Tortorano, {Anna Maria}",

year = "2012",

doi = "10.1128/JCM.00737-12",

language = "English",

volume = "50",

pages = "2472--2476",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology",

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Posteraro, B, Vella, A, Cogliati, M, De Carolis, E, Florio, AR, Posteraro, P, Sanguinetti, M & Tortorano, AM 2012, 'Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii', Journal of Clinical Microbiology, vol. 50, pagg. 2472-2476. https://doi.org/10.1128/JCM.00737-12

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii. / Posteraro, Brunella; Vella, Antonietta; Cogliati, Massimo et al.
In: Journal of Clinical Microbiology, Vol. 50, 2012, pag. 2472-2476.

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

TY - JOUR

T1 - Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

AU - Posteraro, Brunella

AU - Vella, Antonietta

AU - Cogliati, Massimo

AU - De Carolis, Elena

AU - Florio, Ada Rita

AU - Posteraro, Patrizia

AU - Sanguinetti, Maurizio

AU - Tortorano, Anna Maria

PY - 2012

Y1 - 2012

N2 - We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.

AB - We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.

KW - Cryptococcus neoformans

UR - http://hdl.handle.net/10807/13092

U2 - 10.1128/JCM.00737-12

DO - 10.1128/JCM.00737-12

M3 - Article

SN - 1098-660X

VL - 50

SP - 2472

EP - 2476

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

ER -

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii

Abstract

Keywords

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