Lycopene induces apoptosis in immortalized fibroblasts exposed to tobacco smoke condensate through arresting cell cycle and down-regulating cyclin D1, pAKT and pBad.

Simona Serini, Gabriella Calviello, Paola Palozza, Mariana Alessia Sheriff, Alma Boninsegna Lucarelli, Nicola Giuseppe Maggiano, Franco Oreste Ranelletti, Achille Renato Maria Cittadini

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

There is a lot of interest in the health benefits of dietary carotenoids and on the relationship of these compounds with smoke. In particular, it is unknown if the enhanced cancer risk observed in smokers following β-carotene supplementation can be also found using other carotenoids. Here, we studied the effects of the tomato carotenoid lycopene on molecular pathways involved in cell cycle progression, apoptosis and survival in immortalized RAT-1 fibroblasts exposed to cigarette smoke condensate (TAR). Lycopene (0.5 2.0 μM) inhibited cell growth in a dose-and time-dependent manner, by arresting cell cycle progression and by promoting apoptosis in cells exposed to TAR. The arrest of cell cycle was independent of p53 and of 8-OH-dG DNA damage and related to a decreased expression of cyclin D1. Moreover, the carotenoid up-regulated apoptosis and down-regulated the phosphorylation of AKT and Bad in cells exposed to TAR. Such an effect was associated to an inhibition of TAR-induced expression of Cox-2 and hsp90, which is known to maintain AKT activity. This study suggests that lycopene, differently from β-carotene, can exert protective effects against cigarette smoke condensate.
Lingua originaleEnglish
pagine (da-a)1445-1456
Numero di pagine12
RivistaAPOPTOSIS
Stato di pubblicazionePubblicato - 2006

Keywords

  • apoptosis
  • cell cycle
  • cigarette smoke condensate
  • immortalized cells
  • lycopene

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