Low reliability of anti-KIR4.183-120 peptide auto-antibodies in multiple sclerosis patients

Mariapaola Marino, Gabriele Di Sante, Carlo Provenzano, Massimiliano Mirabella, Giovambattista Pani, Francesco Ria, Giovanni Frisullo, Daniela Maria Samengo, Emanuela Bartoccioni

Risultato della ricerca: Contributo in rivistaArticolo in rivista

4 Citazioni (Scopus)

Abstract

Abstract BACKGROUND: Multiple sclerosis (MS) is an autoimmune disease for which auto-antibodies fully validated as diagnostic and prognostic biomarkers are widely desired. Recently, an immunoreactivity against the inward rectifying potassium channel 4.1 (KIR4.1) has been reported in a large proportion of a group of MS patients, with amino acids 83-120 being the major epitope. Moreover, a strong correlation between anti-KIR4.183-120 and anti-full-length-protein auto-antibodies titer was reported. However, this finding received limited confirmation. OBJECTIVE: Validation of the diagnostic potential of anti-KIR4.183-120 antibodies in 78 MS patients, 64 healthy blood donors, and 42 individuals with other neurological diseases. METHODS: Analysis of anti-KIR4.183-120 antibodies by enzyme-linked immunosorbent assay (ELISA) using a mouse antiserum we produced as a new ELISA reliability control. Additionally, evaluation of reactivity against 293-T cells transiently transfected with full-length KIR4.1 by flow cytometry. RESULTS: We found antibodies to KIR4.183-120 only in 13 out of 78 (16.6%) MS patients; among these, only 2 were positive for anti-full-length KIR4.1 antibodies. CONCLUSION: Employing a new reliability control and a new cytofluorometric assay, we cannot support anti-KIR4.183-120 auto-antibodies as a reliable biomarker in MS.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
RivistaMultiple Sclerosis
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • KIR4.1
  • auto-antibodies
  • biomarker
  • enzyme-linked immunosorbent assay
  • flow cytometry
  • multiple sclerosis

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