Abstract
Abstract
Objective: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis.
Design: Experimental study.
Setting: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy.
Patient(s): Cultures of primary decidual cells isolated from human term placenta.
Intervention(s): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities.
Main outcome measure(s): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated.
Result(s): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities.
Conclusion(s): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
Lingua originale | Inglese |
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pagine (da-a) | 169-177 |
Numero di pagine | 9 |
Rivista | Fertility and Sterility |
DOI | |
Stato di pubblicazione | Pubblicato - 2012 |
Keywords
- Anticoagulants
- Apoptosis
- Caspase 3
- Caspase 8
- Cell Survival
- Decidua
- Enoxaparin
- Female
- Gene Expression
- Heparin, Low-Molecular-Weight
- Humans
- In Situ Nick-End Labeling
- Intercellular Signaling Peptides and Proteins
- Pregnancy
- Primary Cell Culture
- Tumor Necrosis Factor-alpha