Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis

Nicoletta Di Simone, Giovanni Scambia, Fiorella Di Nicuolo, Roberta Castellani, Manuela Veglia, Chiara Tersigni, Riccardo Marana, Marco Silano

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

10 Citazioni (Scopus)

Abstract

Abstract Objective: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis. Design: Experimental study. Setting: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy. Patient(s): Cultures of primary decidual cells isolated from human term placenta. Intervention(s): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities. Main outcome measure(s): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated. Result(s): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities. Conclusion(s): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
Lingua originaleEnglish
pagine (da-a)169-177
Numero di pagine9
RivistaFertility and Sterility
DOI
Stato di pubblicazionePubblicato - 2012

Keywords

  • Anticoagulants
  • Apoptosis
  • Caspase 3
  • Caspase 8
  • Cell Survival
  • Decidua
  • Enoxaparin
  • Female
  • Gene Expression
  • Heparin, Low-Molecular-Weight
  • Humans
  • In Situ Nick-End Labeling
  • Intercellular Signaling Peptides and Proteins
  • Pregnancy
  • Primary Cell Culture
  • Tumor Necrosis Factor-alpha

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