TY - JOUR
T1 - Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor-like growth factor expression and promote survival of decidual cells undergoing apoptosis.
AU - Scambia, Giovanni
AU - Di Simone, Nicoletta
AU - Di Nicuolo, Fiorella
AU - Castellani, Roberta
AU - Veglia, Manuela
AU - Silano, Marco
AU - Tersigni, Chiara
AU - Tritarelli, Alessandra
AU - Marana, Riccardo
PY - 2012
Y1 - 2012
N2 - OBJECTIVE: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis.
DESIGN: Experimental study.
SETTING: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy.
PATIENT(S): Cultures of primary decidual cells isolated from human term placenta.
INTERVENTION(S): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities.
MAIN OUTCOME MEASURE(S): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated.
RESULT(S): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities.
CONCLUSION(S): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
AB - OBJECTIVE: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis.
DESIGN: Experimental study.
SETTING: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy.
PATIENT(S): Cultures of primary decidual cells isolated from human term placenta.
INTERVENTION(S): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities.
MAIN OUTCOME MEASURE(S): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated.
RESULT(S): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities.
CONCLUSION(S): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
KW - LOW MOLECULAR WEIGHT HEPARINS
KW - LOW MOLECULAR WEIGHT HEPARINS
UR - http://hdl.handle.net/10807/3751
U2 - 10.1016/j.fertnstert.2011.10.021
DO - 10.1016/j.fertnstert.2011.10.021
M3 - Article
SN - 0015-0282
VL - 97
SP - 169
EP - 177
JO - Fertility and Sterility
JF - Fertility and Sterility
ER -