OBJECTIVE: To evaluate the effects of low-molecular-weight heparins (LMWHs) on decidual heparin-binding epidermal growth factor-like growth factor (HB-EGF) expression/secretion and on TNF-α-induced decidual apoptosis.
DESIGN: Experimental study.
SETTING: Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore, Rome, Italy.
PATIENT(S): Cultures of primary decidual cells isolated from human term placenta.
INTERVENTION(S): The effects of LMWHs (tinzaparin and enoxaparin) on decidual HB-EGF expression and secretion were investigated by Western blot analysis and ELISA, respectively. TNF-α-induced decidual apoptosis was evaluated by annexin V staining, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay, and caspase activities.
MAIN OUTCOME MEASURE(S): Decidual HB-EGF expression/secretion and apoptotic rate induced by TNF-α were investigated.
RESULT(S): Tinzaparin enhanced decidual HB-EGF expression and secretion. TNF-α reduced the number of viable cells by inducing apoptosis. Simultaneous addition of LMWHs (primarily tinzaparin) blocked the increase in annexin V- and TUNEL-positive cells and reduced the amount of caspase activities.
CONCLUSION(S): Both LMWHs induced a significant increase in decidual HB-EGF expression/secretion and reduced TNF-α-induced decidual apoptosis. Tinzaparin demonstrated higher efficacy.
|Numero di pagine||9|
|Rivista||Fertility and Sterility|
|Stato di pubblicazione||Pubblicato - 2012|
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