Local structure and dynamics of hemeproteins by X-ray absorption near edge structure spectroscopy.

Alessandro Arcovito, 33182, DI MEDICINA E CHIRURGIA "A.GEMELLI" FACOLTA', cliniche intensivologiche e perioperatorie ROMA - Dipartimento di Scienze biotecnologiche di base, Stefano Della Longa

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

X-ray absorption near edge structure (XANES) spectroscopy is a synchrotron radiation technique sensitive to the local structure and dynamics around the metal site of a heme containing protein. Advances in detection techniques and theoretical/computational platforms in the last 15years allowed the use of XANES as a quantitative probe of the key structural determinants driving functional changes, both in a concerted way with protein crystallography and EXAFS (extended X-ray absorption fine structure), or as a stand-alone method to apply in the crystal state as well as in solution. Moreover, the local dynamics of the heme site has been deeply investigated, on one hand, coupling XANES to classical photolysis experiments at cryogenic temperatures; on the other hand, the intrinsic property of the synchrotron radiation to induce radiolysis events, has been exploited to investigate specific cryotrapped intermediates, using X-rays both as a pump and a probe. Insights on the XANES method and some specific examples are presented to illustrate these topics.
Lingua originaleEnglish
pagine (da-a)93-99
Numero di pagine7
RivistaJournal of Inorganic Biochemistry
DOI
Stato di pubblicazionePubblicato - 2012

Keywords

  • Photolysis
  • Structural Dynamics

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