TY - JOUR
T1 - Liquid chromatography-mass spectrometry measurement of leukotrienes in asthma and other respiratory diseases
AU - Montuschi, Paolo
AU - Santini, Giuseppe
AU - Valente, Salvatore
AU - Mondino, Chiara
AU - Macagno, Francesco
AU - Cattani Franchi, Paola
AU - Zini Tanzi, Gina
AU - Mores, Nadia
PY - 2014
Y1 - 2014
N2 - Leukotrienes (LTs), including cysteinyl-LTs (LTC4, LTD4 and LTE4) and LTB4, are potent inflammatory lipid mediators which have been involved in the pathophysiology of respiratory diseases. LC-MS/MS techniques for measuring LT concentrations in sputum supernatants, serum, urine and exhaled breath condensate (EBC) have been developed. In asthmatic adults, reported LTB4 and LTE4 concentrations in sputum range from 79 to 7220pg/ml and from 11.9 to 891pg/ml, respectively. Data on sputum LT concentrations in healthy subjects are not available. In EBC, reported LTE4 concentrations range from 38 to 126pg/ml (95% CI) in adult asthma patients and from 34 to 48pg/ml in healthy subjects. LTB4 concentrations in EBC range from 175 to 315pg/ml (interquartile range) in asthmatic children, and from 25 to 245pg/ml in healthy children. Enabling an accurate quantitative assessment of LTs in biological fluids, LC-MS/MS techniques provide a valuable tool for exploring the pathophysiological role of LTs in respiratory disease and might be useful for assessing the effects of therapeutic intervention. This review presents the analytical aspects of the LC-MS/MS techniques for measuring LT concentrations in biological fluids and discusses their potential utility for the assessment of airway inflammation and monitoring of pharmacological treatment in patients with asthma phenotypes and other respiratory diseases.
AB - Leukotrienes (LTs), including cysteinyl-LTs (LTC4, LTD4 and LTE4) and LTB4, are potent inflammatory lipid mediators which have been involved in the pathophysiology of respiratory diseases. LC-MS/MS techniques for measuring LT concentrations in sputum supernatants, serum, urine and exhaled breath condensate (EBC) have been developed. In asthmatic adults, reported LTB4 and LTE4 concentrations in sputum range from 79 to 7220pg/ml and from 11.9 to 891pg/ml, respectively. Data on sputum LT concentrations in healthy subjects are not available. In EBC, reported LTE4 concentrations range from 38 to 126pg/ml (95% CI) in adult asthma patients and from 34 to 48pg/ml in healthy subjects. LTB4 concentrations in EBC range from 175 to 315pg/ml (interquartile range) in asthmatic children, and from 25 to 245pg/ml in healthy children. Enabling an accurate quantitative assessment of LTs in biological fluids, LC-MS/MS techniques provide a valuable tool for exploring the pathophysiological role of LTs in respiratory disease and might be useful for assessing the effects of therapeutic intervention. This review presents the analytical aspects of the LC-MS/MS techniques for measuring LT concentrations in biological fluids and discusses their potential utility for the assessment of airway inflammation and monitoring of pharmacological treatment in patients with asthma phenotypes and other respiratory diseases.
KW - Airway inflammation
KW - Asthma
KW - Biomarkers
KW - Exhaled breath condensate
KW - Leukotrienes
KW - Liquid chromatography–mass spectrometry
KW - Respiratory medicine
KW - Airway inflammation
KW - Asthma
KW - Biomarkers
KW - Exhaled breath condensate
KW - Leukotrienes
KW - Liquid chromatography–mass spectrometry
KW - Respiratory medicine
UR - http://hdl.handle.net/10807/57340
U2 - 10.1016/j.jchromb.2014.02.059
DO - 10.1016/j.jchromb.2014.02.059
M3 - Article
SN - 1873-376X
VL - 964
SP - 12
EP - 25
JO - JOURNAL OF CHROMATOGRAPHY B
JF - JOURNAL OF CHROMATOGRAPHY B
ER -