LINC00174 is a novel prognostic factor in thymic epithelial tumors involved in cell migration and lipid metabolism

Silvia Masciarelli, Claudia Tito, Federica Ganci, Andrea Sacconi, Giulia Fontemaggi, Claudio Pulito, Enzo Gallo, Valentina Laquintana, Alessia Iaiza, Luciana De Angelis, Anna Benedetti, Jessica Cacciotti, Selenia Miglietta, Maria Bellenghi, Alessandra Carè, Alessandro Fatica, Daniele Diso, Marco Anile, Vincenzo Petrozza, Francesco FaccioloGabriele Alessandrini, Edoardo Pescarmona, Federico Venuta, Mirella Marino, Giovanni Blandino, Francesco Fazi

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

1 Citazioni (Scopus)

Abstract

Long non-coding RNAs are emerging as new molecular players involved in many biological processes, such as proliferation, apoptosis, cell cycle, migration, and differentiation. Their aberrant expression has been reported in variety of diseases. The aim of this study is the identification and functional characterization of clinically relevant lncRNAs responsible for the inhibition of miR-145-5p, a key tumor suppressor in thymic epithelial tumors (TETs). Starting from gene expression analysis by microarray in a cohort of fresh frozen thymic tumors and normal tissues, we identified LINC00174 as upregulated in TET. Interestingly, LINC00174 expression is positively correlated with a 5-genes signature in TETs. Survival analyses, performed on the TCGA dataset, showed that LINC00174 and its associated 5-genes signature are prognostic in TETs. Specifically, we show that LINC00174 favors the expression of SYBU, FEM1B, and SCD5 genes by sponging miR-145-5p, a well-known tumor suppressor microRNA downregulated in a variety of tumors, included TETs. Functionally, LINC00174 impacts on cell migration and lipid metabolism. Specifically, SCD5, one of the LINC00174-associated genes, is implicated in the control of lipid metabolism and promotes thymic cancer cells migration. Our study highlights that LINC00174 and its associated gene signature are relevant prognostic indicators in TETs. Of note, we here show that a key controller of lipid metabolism, SCD5, augments the migration ability of TET cells, creating a link between lipids and motility, and highlighting these pathways as relevant targets for the development of novel therapeutic approaches for TET.
Lingua originaleEnglish
pagine (da-a)959-N/A
RivistaCELL DEATH & DISEASE
Volume11
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • differentiation
  • non coding RNA

Fingerprint

Entra nei temi di ricerca di 'LINC00174 is a novel prognostic factor in thymic epithelial tumors involved in cell migration and lipid metabolism'. Insieme formano una fingerprint unica.

Cita questo