Lack of relationship between genotype and virulence in Candida species

Judith Díaz-García; Maiken C. Arendrup; Rafael Cantón; Julio García-Rodríguez; Elia Gómez García De La Pedrosa; Gabriella Parisi; Javier Pemán; Brunella Posteraro; Maurizio Sanguinetti; Daniel Archimedes Da Matta; Arnaldo L. Colombo; Patricia Muñoz; Carlos Sánchez-Carrillo; Jesús Guinea; Pilar Escribano

doi:10.1016/j.riam.2020.10.002

Lack of relationship between genotype and virulence in Candida species

Judith Díaz-García
, Maiken C. Arendrup
, Rafael Cantón
, Julio García-Rodríguez
, Elia Gómez García De La Pedrosa
, Gabriella Parisi
, Javier Pemán
, Brunella Posteraro
, Maurizio Sanguinetti
, Daniel Archimedes Da Matta
, Arnaldo L. Colombo
, Patricia Muñoz
, Carlos Sánchez-Carrillo
, Jesús Guinea
, Pilar Escribano

Hospital General Universitario Gregorio Marañon
Statens Serum Institut
Hospital Ramon y Cajal
Universidad Autónoma de Madrid
San Camillo Hospital
Instituto Investigacion Sanitaria la Fe
Universidade Federal de São Paulo

Risultato della ricerca: Contributo in rivista › Articolo

Abstract

Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

Titolo tradotto del contributo	[Autom. eng. transl.] Lack of relationship between genotype and virulence in Candida species
Lingua originale	Spagnolo
pagine (da-a)	N/A-N/A
Rivista	Revista Iberoamericana de Micologia
DOI	https://doi.org/10.1016/j.riam.2020.10.002
Stato di pubblicazione	Pubblicato - 2020

Keywords

Candida albicans
Candida parapsilosis
Candida tropicalis
Galleria mellonella
Genotyping
Virulence

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10.1016/j.riam.2020.10.002

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Díaz-García, J., Arendrup, M. C., Cantón, R., García-Rodríguez, J., De La Pedrosa, E. G. G., Parisi, G., Pemán, J., Posteraro, B., Sanguinetti, M., Da Matta, D. A., Colombo, A. L., Muñoz, P., Sánchez-Carrillo, C., Guinea, J., & Escribano, P. (2020). Lack of relationship between genotype and virulence in Candida species. Revista Iberoamericana de Micologia, N/A-N/A. https://doi.org/10.1016/j.riam.2020.10.002

@article{dfb7010071724a89977647c198014ac2,

title = "Lack of relationship between genotype and virulence in Candida species",

abstract = "Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5{\textendash}2 vs. 2 days, IQR 1{\textendash}2.25), C. parapsilosis (2 days, IQR 1.5{\textendash}2.6 vs. 2 days, IQR 2{\textendash}3.3), and C. tropicalis (1 day, IQR 1{\textendash}3.5 vs. 2 days, IQR 2{\textendash}3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.",

keywords = "Candida albicans, Candida parapsilosis, Candida tropicalis, Galleria mellonella, Genotyping, Virulence, Candida albicans, Candida parapsilosis, Candida tropicalis, Galleria mellonella, Genotyping, Virulence",

author = "Judith D{\'i}az-Garc{\'i}a and Arendrup, \{Maiken C.\} and Rafael Cant{\'o}n and Julio Garc{\'i}a-Rodr{\'i}guez and \{De La Pedrosa\}, \{Elia G{\'o}mez Garc{\'i}a\} and Gabriella Parisi and Javier Pem{\'a}n and Brunella Posteraro and Maurizio Sanguinetti and \{Da Matta\}, \{Daniel Archimedes\} and Colombo, \{Arnaldo L.\} and Patricia Mu{\~n}oz and Carlos S{\'a}nchez-Carrillo and Jes{\'u}s Guinea and Pilar Escribano",

year = "2020",

doi = "10.1016/j.riam.2020.10.002",

language = "Spagnolo",

pages = "N/A--N/A",

journal = "Revista Iberoamericana de Micologia",

issn = "1130-1406",

publisher = "Asociacion Espanola de Micologia",

}

Díaz-García, J, Arendrup, MC, Cantón, R, García-Rodríguez, J, De La Pedrosa, EGG, Parisi, G, Pemán, J, Posteraro, B , Sanguinetti, M, Da Matta, DA, Colombo, AL, Muñoz, P, Sánchez-Carrillo, C, Guinea, J & Escribano, P 2020, 'Lack of relationship between genotype and virulence in Candida species', Revista Iberoamericana de Micologia, pagg. N/A-N/A. https://doi.org/10.1016/j.riam.2020.10.002

TY - JOUR

T1 - Lack of relationship between genotype and virulence in Candida species

AU - Díaz-García, Judith

AU - Arendrup, Maiken C.

AU - Cantón, Rafael

AU - García-Rodríguez, Julio

AU - De La Pedrosa, Elia Gómez García

AU - Parisi, Gabriella

AU - Pemán, Javier

AU - Posteraro, Brunella

AU - Sanguinetti, Maurizio

AU - Da Matta, Daniel Archimedes

AU - Colombo, Arnaldo L.

AU - Muñoz, Patricia

AU - Sánchez-Carrillo, Carlos

AU - Guinea, Jesús

AU - Escribano, Pilar

PY - 2020

Y1 - 2020

N2 - Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

AB - Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

KW - Candida albicans

KW - Candida parapsilosis

KW - Candida tropicalis

KW - Galleria mellonella

KW - Genotyping

KW - Virulence

KW - Candida albicans

KW - Candida parapsilosis

KW - Candida tropicalis

KW - Galleria mellonella

KW - Genotyping

KW - Virulence

UR - http://hdl.handle.net/10807/166933

U2 - 10.1016/j.riam.2020.10.002

DO - 10.1016/j.riam.2020.10.002

M3 - Articolo

SN - 1130-1406

SP - N/A-N/A

JO - Revista Iberoamericana de Micologia

JF - Revista Iberoamericana de Micologia

ER -

Lack of relationship between genotype and virulence in Candida species

Abstract

Keywords

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