TY - JOUR
T1 - Lack of relationship between genotype and virulence in Candida species
AU - Díaz-García, Judith
AU - Arendrup, Maiken C.
AU - Cantón, Rafael
AU - García-Rodríguez, Julio
AU - De La Pedrosa, Elia Gómez García
AU - Parisi, Gabriella
AU - Pemán, Javier
AU - Posteraro, Brunella
AU - Sanguinetti, Maurizio
AU - Da Matta, Daniel Archimedes
AU - Colombo, Arnaldo L.
AU - Muñoz, Patricia
AU - Sánchez-Carrillo, Carlos
AU - Guinea, Jesús
AU - Escribano, Pilar
PY - 2020
Y1 - 2020
N2 - Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.
AB - Background: The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied. Aims: We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model. Methods: One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae. Results: The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5–2 vs. 2 days, IQR 1–2.25), C. parapsilosis (2 days, IQR 1.5–2.6 vs. 2 days, IQR 2–3.3), and C. tropicalis (1 day, IQR 1–3.5 vs. 2 days, IQR 2–3.5; p < 0.05). High intra-cluster variability in terms of median of survival was found regardless the species. Conclusions: No relationship between genotype and virulence in Candida was observed with the G. mellonella model.
KW - Candida albicans
KW - Candida parapsilosis
KW - Candida tropicalis
KW - Galleria mellonella
KW - Genotyping
KW - Virulence
KW - Candida albicans
KW - Candida parapsilosis
KW - Candida tropicalis
KW - Galleria mellonella
KW - Genotyping
KW - Virulence
UR - http://hdl.handle.net/10807/166933
U2 - 10.1016/j.riam.2020.10.002
DO - 10.1016/j.riam.2020.10.002
M3 - Articolo in rivista
SN - 1130-1406
SP - N/A-N/A
JO - Revista Iberoamericana de Micologia
JF - Revista Iberoamericana de Micologia
ER -