Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

D. Voccia; Diego Voccia; M. Sosnowska; F. Bettazzi; I. Palchetti; W. Kutner

doi:10.1007/978-3-319-09617-9_11

Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

D. Voccia, Diego Voccia, M. Sosnowska, F. Bettazzi, I. Palchetti, W. Kutner

Risultato della ricerca: Contributo in libro › Contributo a convegno

1 Citazioni (Scopus)

Abstract

Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.

Lingua originale	English
Titolo della pubblicazione ospite	Lecture Notes in Electrical Engineering
Pagine	59-64
Numero di pagine	6
Volume	319
DOI	https://doi.org/10.1007/978-3-319-09617-9_11
Stato di pubblicazione	Pubblicato - 2015
Evento	2nd National Conference on Sensors, 2014 - ita Durata: 19 feb 2014 → 21 feb 2014

Serie di pubblicazioni

Nome	LECTURE NOTES IN ELECTRICAL ENGINEERING

Convegno

Convegno	2nd National Conference on Sensors, 2014
Città	ita
Periodo	19/2/14 → 21/2/14

Keywords

Electropolymerization

Accesso al documento

10.1007/978-3-319-09617-9_11

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@inproceedings{3779d00c5e0b476b895a4c087037141e,

title = "Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes",

abstract = "Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.",

keywords = "Electropolymerization, Electropolymerization",

author = "D. Voccia and Diego Voccia and M. Sosnowska and F. Bettazzi and I. Palchetti and W. Kutner",

year = "2015",

doi = "10.1007/978-3-319-09617-9_11",

language = "English",

isbn = "978-3-319-09616-2",

volume = "319",

series = "LECTURE NOTES IN ELECTRICAL ENGINEERING",

pages = "59--64",

booktitle = "Lecture Notes in Electrical Engineering",

note = "2nd National Conference on Sensors, 2014 ; Conference date: 19-02-2014 Through 21-02-2014",

}

Voccia, D, Voccia, D, Sosnowska, M, Bettazzi, F, Palchetti, I & Kutner, W 2015, Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes. in Lecture Notes in Electrical Engineering. vol. 319, LECTURE NOTES IN ELECTRICAL ENGINEERING, pagg. 59-64, 2nd National Conference on Sensors, 2014, ita, 19/2/14. https://doi.org/10.1007/978-3-319-09617-9_11

TY - GEN

T1 - Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

AU - Voccia, D.

AU - Voccia, Diego

AU - Sosnowska, M.

AU - Bettazzi, F.

AU - Palchetti, I.

AU - Kutner, W.

PY - 2015

Y1 - 2015

N2 - Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.

AB - Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.

KW - Electropolymerization

UR - http://hdl.handle.net/10807/155920

U2 - 10.1007/978-3-319-09617-9_11

DO - 10.1007/978-3-319-09617-9_11

M3 - Conference contribution

SN - 978-3-319-09616-2

VL - 319

T3 - LECTURE NOTES IN ELECTRICAL ENGINEERING

SP - 59

EP - 64

BT - Lecture Notes in Electrical Engineering

T2 - 2nd National Conference on Sensors, 2014

Y2 - 19 February 2014 through 21 February 2014

ER -

Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

Abstract

Serie di pubblicazioni

Convegno

Keywords

Accesso al documento

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