Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes

D. Voccia, Diego Voccia, M. Sosnowska, F. Bettazzi, I. Palchetti, W. Kutner

Risultato della ricerca: Contributo in libroContributo a convegno

1 Citazioni (Scopus)

Abstract

Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
Lingua originaleEnglish
Titolo della pubblicazione ospiteLecture Notes in Electrical Engineering
Pagine59-64
Numero di pagine6
Volume319
DOI
Stato di pubblicazionePubblicato - 2015
Evento2nd National Conference on Sensors, 2014 - ita
Durata: 19 feb 201421 feb 2014

Serie di pubblicazioni

NomeLECTURE NOTES IN ELECTRICAL ENGINEERING

Convegno

Convegno2nd National Conference on Sensors, 2014
Cittàita
Periodo19/2/1421/2/14

Keywords

  • Electropolymerization

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