TY - GEN
T1 - Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes
AU - Voccia, D.
AU - Voccia, Diego
AU - Sosnowska, M.
AU - Bettazzi, F.
AU - Palchetti, I.
AU - Kutner, W.
PY - 2015
Y1 - 2015
N2 - Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
AB - Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
KW - Electropolymerization
KW - Electropolymerization
UR - http://hdl.handle.net/10807/155920
U2 - 10.1007/978-3-319-09617-9_11
DO - 10.1007/978-3-319-09617-9_11
M3 - Conference contribution
SN - 978-3-319-09616-2
VL - 319
T3 - LECTURE NOTES IN ELECTRICAL ENGINEERING
SP - 59
EP - 64
BT - Lecture Notes in Electrical Engineering
T2 - 2nd National Conference on Sensors, 2014
Y2 - 19 February 2014 through 21 February 2014
ER -