Isolation, culture, and phenotypic characterization of mesenchymal stromal cells from the amniotic membrane of human term placenta

Ornella Parolini, Marta Magatti, Stefano Pianta, Antonietta Silini

Risultato della ricerca: Contributo in libroChapter

20 Citazioni (Scopus)

Abstract

During the past several years, the human placenta and in particular the amniotic fetal membrane have attracted much attention as a possible source of cells to be used in cell therapy approaches due to its putative stem cell potential associated with its early embryonic origin and its immunomodulatory potential associated with its role in fetomaternal tolerance. Within the human amniotic membrane, it is possible to isolate two main cell populations: amniotic epithelial cells (from the epithelial layer of the amniotic membrane) and amniotic mesenchymal cells (from the mesenchymal layer of the amniotic membrane). In this chapter, we will describe a method for the isolation of mesenchymal stromal cells (hAMSCs). We will also describe their optimal culture conditions and the phenotypic characterization of cells after passaging.
Lingua originaleEnglish
Titolo della pubblicazione ospiteMesenchymal Stem Cells - Methods and Protocols, Second Edition
Pagine233-244
Numero di pagine12
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Amniotic membrane
  • Cell isolation
  • Chang Medium ® C
  • Genetics
  • Human term placenta
  • Mesenchymal stromal cells
  • Molecular Biology
  • Phenotype

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