TY - JOUR
T1 - Involvement of peptidylprolyl cis/trans isomerases in Enterococcus faecalis virulence
AU - Reffuveille, F
AU - Connil, N
AU - Sanguinetti, Maurizio
AU - Posteraro, Brunella
AU - Chevalier, S
AU - Auffray, Y
AU - Rince, A.
PY - 2012
Y1 - 2012
N2 - Peptidylprolyl cis/trans isomerases (PPIases) are enzymes involved in protein folding. Analysis of the genome sequence of Enterococcus faecalis V583 allowed for identification of 3 PPIases carrying genes. ef2898 encodes an intracellular PPIase which was not shown to be important for the E. faecalis stress response or virulence. The other two PPIases, the parvulin family rotamase EF0685 and the cyclophilin family member EF1534, are expected to be surface-exposed proteins. They were shown to be important for virulence and resistance to NaCl. A Δef0685 Δef1534 mutant was also more resistant to oxidative stress, was able to grow under a high manganese concentration, and showed altered resistance to ampicillin and quinolone antibiotics.
AB - Peptidylprolyl cis/trans isomerases (PPIases) are enzymes involved in protein folding. Analysis of the genome sequence of Enterococcus faecalis V583 allowed for identification of 3 PPIases carrying genes. ef2898 encodes an intracellular PPIase which was not shown to be important for the E. faecalis stress response or virulence. The other two PPIases, the parvulin family rotamase EF0685 and the cyclophilin family member EF1534, are expected to be surface-exposed proteins. They were shown to be important for virulence and resistance to NaCl. A Δef0685 Δef1534 mutant was also more resistant to oxidative stress, was able to grow under a high manganese concentration, and showed altered resistance to ampicillin and quinolone antibiotics.
KW - Amino Acid Sequence
KW - Animals
KW - Bacterial
KW - Bacterial Proteins
KW - Enterococcus faecalis
KW - Enzymologic
KW - Female
KW - Gene Expression Regulation
KW - Genetic Complementation Test
KW - Gram-Positive Bacterial Infections
KW - Inbred BALB C
KW - Mice
KW - Molecular Sequence Data
KW - Mutation
KW - Peptidylprolyl Isomerase
KW - Virulence
KW - Amino Acid Sequence
KW - Animals
KW - Bacterial
KW - Bacterial Proteins
KW - Enterococcus faecalis
KW - Enzymologic
KW - Female
KW - Gene Expression Regulation
KW - Genetic Complementation Test
KW - Gram-Positive Bacterial Infections
KW - Inbred BALB C
KW - Mice
KW - Molecular Sequence Data
KW - Mutation
KW - Peptidylprolyl Isomerase
KW - Virulence
UR - https://publicatt.unicatt.it/handle/10807/18291
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84861169830&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861169830&origin=inward
U2 - 10.1128/IAI.06251-11
DO - 10.1128/IAI.06251-11
M3 - Article
SN - 0019-9567
VL - 80
SP - 1728
EP - 1735
JO - Infection and Immunity
JF - Infection and Immunity
IS - 5
ER -