Involvement of Enterococcus faecalis small RNAs in stress response and virulence

Maurizio Sanguinetti, Cecilia Martini, Charlotte Michaux, Axel Hartke, Swantje Reiss, Dirk Albrecht, Aurélie Budin-Verneuil, Susanne Engelmann, Torsten Hain, Nicolas Verneuil, Jean-Christophe Giard

Risultato della ricerca: Contributo in rivistaArticolo in rivista

31 Citazioni (Scopus)

Abstract

Candidate small RNAs (sRNAs) have recently been identified in Enterococcus faecalis, a Gram-positive opportunistic pathogen, and six of these candidate sRNAs with unknown functions were selected for a functional study. Deletion mutants and complemented strains were constructed, and their virulence was tested. We were unable to obtain the ef0869-0870 mutant, likely due to an essential role, and the ef0820-0821 sRNA seemed not to be involved in virulence. In contrast, the mutant lacking ef0408-0409 sRNA, homologous to the RNAII component of the toxin-antitoxin system, appeared more virulent and more able to colonize mouse organs. The three other mutants showed reduced virulence. In addition, we checked the responses of these mutant strains to several stresses encountered in the gastrointestinal tract or during the infection process. In parallel, the activities of the sRNA promoters were measured using transcriptional fusion constructions. To attempt to identify the regulons of these candidate sRNAs, proteomics profiles of the mutant strains were compared with that of the wild type. This showed that the selected sRNAs controlled the expression of proteins involved in diverse cellular processes and the stress response. The combined data highlight the roles of certain candidate sRNAs in the adaptation of E. faecalis to environmental changes and in the complex transition process from a commensal to a pathogen.
Lingua originaleEnglish
pagine (da-a)3599-3611
Numero di pagine13
RivistaInfection and Immunity
Volume82
DOI
Stato di pubblicazionePubblicato - 2014

Keywords

  • Animals
  • Enterococcus faecalis
  • Female
  • Gastrointestinal Tract
  • Gene Expression Regulation, Bacterial
  • Gram-Positive Bacterial Infections
  • Macrophages
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Promoter Regions, Genetic
  • RNA, Bacterial
  • Stress, Physiological
  • Virulence

Fingerprint Entra nei temi di ricerca di 'Involvement of Enterococcus faecalis small RNAs in stress response and virulence'. Insieme formano una fingerprint unica.

Cita questo