Investigation of the membrane fluidity regulation of fatty acid intracellular distribution by fluorescence lifetime imaging of novel polarity sensitive fluorescent derivatives

Marco De Spirito, Giuseppe Maulucci, Giada Bianchetti, Salome Azoulay-Ginsburg, Nimrod Yosef Keshet-Levy, Aviv Malka, Sofia Zilber, Edward E. Korshin, Shlomo Sasson, Arie Gruzman

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

Free fatty acids are essential structural components of the cell, and their intracellular distribution and effects on membrane organelles have crucial roles in regulating the metabolism, development, and cell cycle of most cell types. Here we engineered novel fluorescent, polarity-sensitive fatty acid derivatives, with the fatty acid aliphatic chain of increasing length (from 12 to 18 carbons). As in the laurdan probe, the lipophilic acyl tail is connected to the environmentally sensitive dimethylaminonaphthalene moiety. The fluorescence lifetime imaging analysis allowed us to monitor the intracellular distribution of the free fatty acids within the cell, and to simultaneously examine how the fluidity and the microviscosity of the membrane environment influence their localization. Each of these probes can thus be used to investigate the membrane fluidity regulation of the correspondent fatty acid intracellular distribution. We observed that, in PC-12 cells, fluorescent sensitive fatty acid derivatives with increased chain length compartmentalize more preferentially in the fluid regions, characterized by a low microviscosity. Moreover, fatty acid derivatives with the longest chain compartmentalize in lipid droplets and lysosomes with characteristic lifetimes, thus making these probes a promising tool for monitoring lipophagy and related events.
Lingua originaleEnglish
pagine (da-a)1-17
Numero di pagine17
RivistaInternational Journal of Molecular Sciences
Volume22
DOI
Stato di pubblicazionePubblicato - 2021

Keywords

  • 2-Naphthylamine
  • Animals
  • FLIM
  • Fatty Acids
  • Fatty acids analogs
  • Fluorescence
  • Fluorescent Dyes
  • Intracellular Space
  • Laurates
  • Laurdan derivatives
  • Lysosomes
  • Membrane Fluidity
  • Membrane fluidity
  • PC12 Cells
  • Phasor analysis
  • Rats
  • Solvents
  • Two-photons mi-croscopy
  • Viscosity

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