TY - JOUR
T1 - Intracellular concentration map of magnesium in whole cells by combined use of x-ray fluorescence microscopy and atomic force microscopy
AU - Lagomarsino, S
AU - Iotti, S
AU - Farruggia, G
AU - Cedola, A
AU - Trapani, Valentina
AU - Fratini, M
AU - Bukreeva, I
AU - Notargiacomo, A
AU - Mastrototaro, L
AU - Marraccini, C
AU - Sorrentino, A
AU - Mcnulty, I
AU - Vogt, S
AU - Legnini, D
AU - Kim, S
AU - Gianoncelli, A
AU - Maier, J. A. M
AU - Wolf Minotti, Federica
PY - 2011
Y1 - 2011
N2 - We report a novel experimental approach to derive quantitative concentration map of light elements in whole cells by combining two complementary nano-probe methods: X-ray fluorescence microscopy
(XRFM) and atomic force microscopy (AFM). The concentration is derived by normalizing point-by-point the elemental (here Mg) spatial distribution obtained by XRFM, by the thickness measured using AFM. The
considerable difference between the elemental distribution and the concentration maps indicates that this
procedure is essential to obtain reliable information on the role and function of elements in whole cells
AB - We report a novel experimental approach to derive quantitative concentration map of light elements in whole cells by combining two complementary nano-probe methods: X-ray fluorescence microscopy
(XRFM) and atomic force microscopy (AFM). The concentration is derived by normalizing point-by-point the elemental (here Mg) spatial distribution obtained by XRFM, by the thickness measured using AFM. The
considerable difference between the elemental distribution and the concentration maps indicates that this
procedure is essential to obtain reliable information on the role and function of elements in whole cells
KW - atomic force microscopy
KW - cell element concentration
KW - magnesium
KW - synchrotron radiation
KW - x-ray fluorescence microscopy
KW - atomic force microscopy
KW - cell element concentration
KW - magnesium
KW - synchrotron radiation
KW - x-ray fluorescence microscopy
UR - http://hdl.handle.net/10807/31526
M3 - Article
SN - 0584-8547
SP - 834
EP - 840
JO - Spectrochimica Acta, Part B: Atomic Spectroscopy
JF - Spectrochimica Acta, Part B: Atomic Spectroscopy
ER -