Abstract
We report a novel experimental approach to derive quantitative concentration map of light elements in whole cells by combining two complementary nano-probe methods: X-ray fluorescence microscopy
(XRFM) and atomic force microscopy (AFM). The concentration is derived by normalizing point-by-point the elemental (here Mg) spatial distribution obtained by XRFM, by the thickness measured using AFM. The
considerable difference between the elemental distribution and the concentration maps indicates that this
procedure is essential to obtain reliable information on the role and function of elements in whole cells
Lingua originale | English |
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pagine (da-a) | 834-840 |
Numero di pagine | 7 |
Rivista | Spectrochimica Acta, Part B: Atomic Spectroscopy |
Stato di pubblicazione | Pubblicato - 2011 |
Keywords
- atomic force microscopy
- cell element concentration
- magnesium
- synchrotron radiation
- x-ray fluorescence microscopy