Interleukin-27 is a potential marker for the onset of post-transplant malignancies

Giuseppe Grandaliano, Paola Pontrelli, Federica Rascio, Gianluigi Zaza, Matteo Accetturo, Simona Simone, Barbara Infante, Lucrezia Furian, Giuseppe Castellano, Pasquale Ditonno, Michele Battaglia, Luigi Cormio, Giuseppe Carrieri, Antonio Lupo, Paolo Rigotti, Loreto Gesualdo, Giovanni Stallone

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

3 Citazioni (Scopus)

Abstract

Background Malignancies represent the third leading cause of post-transplant mortality worldwide. The main challenge for transplant physicians is a timely diagnosis of this condition. The aim of the study was to identify a soluble diagnostic marker for monitoring the development of post-transplant malignancies. Methods This is a multicentre, observational, perspective, case-control study. We enrolled 47 patients with post-transplant solid neoplasia. As a control group we employed 106 transplant recipients without a history of neoplasia and matched them with cases for the main demographic and clinical features. We investigated the transcriptomic profiles of peripheral blood mononuclear cells from kidney graft recipients with and without post-transplant malignancies enrolled in two of the participating centres, randomly selected from the whole study population. Microarray results were confirmed by quantitative polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) in the remaining patients from the same transplant centres and validated in a further independent group enrolled in two different transplant centres. Results We identified 535 differentially expressed genes comparing patients with and without post-transplant malignancies (fold change ≥2.5; false discovery rate <5%). The cancer pathway was closely related to gene expression data, and one of the most down-regulated genes in this pathway was interleukin-27 (IL-27), a cytokine regulating anti-tumour immunity. Quantitative PCR and ELISA confirmed the microarray data. Interestingly, IL-27 plasma levels were able to discriminate patients with post-transplant neoplasia with a specificity of 80% and a sensitivity of 81%. This observation was confirmed in an independent set of patients from two different transplant centres. Conclusions Our data suggest that IL-27 may represent a potential immunological marker for the timely identification of post-transplant neoplasia.
Lingua originaleEnglish
pagine (da-a)157-166
Numero di pagine10
RivistaNephrology Dialysis Transplantation
Volume34
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Age of Onset
  • Aged
  • Biomarkers
  • Case-Control Studies
  • Female
  • Humans
  • Interleukins
  • Kidney Transplantation
  • Leukocytes, Mononuclear
  • Male
  • Middle Aged
  • Neoplasms
  • Postoperative Complications
  • Prognosis
  • Transcriptome
  • Transplant Recipients
  • gene expression
  • immunology
  • interleukin-27
  • lymphomonocytes
  • post-transplant malignancies

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