Interaction of EBV latent origin of replication with the nuclear matrix: Identification of S/MAR sequences and protein components

Silvia Masciarelli, Giulia Mearini, Silvia Chichiarelli, Michele Zampieri, Maria D'Erme, Anna Ferraro, Elena Mattia

Risultato della ricerca: Contributo in rivistaArticolo in rivista

6 Citazioni (Scopus)

Abstract

During latency, Epstein Barr virus (EBV) genome, as an episome, is attached to the nuclear matrix (NM) via the latent origin of replication ori P. Within this element, we have found that a region, 580 bp long, encompassing the replicator DS element, shows the strongest affinity for the NM. In addition, by cross-linking with cis-diamminedichloroplatinum, we have identified two NM proteins with an apparent molecular weight of 85 and 60 kDa that, with high affinity and specificity, bind ori P. These proteins are not induced by EBV infection, but their interaction with ori P is lost upon induction of EBV lytic cycle. These data strongly suggest that the binding of ori P to specific components of the NM is required for EBV latent replication. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Lingua originaleEnglish
pagine (da-a)119-124
Numero di pagine6
RivistaFEBS Letters
Volume547
DOI
Stato di pubblicazionePubblicato - 2003

Keywords

  • Binding Sites
  • Burkitt Lymphoma
  • Cell Line
  • Cross-linking
  • DNA Primers
  • DNA, Viral
  • Epstein Barr virus
  • Genome, Viral
  • Herpesvirus 4, Human
  • Humans
  • Nuclear Matrix
  • Nuclear matrix
  • Polymerase Chain Reaction
  • Replication Origin
  • Restriction Mapping
  • S/MAR

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