Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

Elisabetta Meucci; Alvaro Mordente

Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

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Abstract

Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

Lingua originale	English
pagine (da-a)	231-238
Numero di pagine	8
Rivista	Italian Journal of Biochemistry
Volume	32
Stato di pubblicazione	Pubblicato - 1983

Keywords

Alkaline Phosphatase
Animals
Ascorbic Acid
Cattle
Kidney
Spectrometry, Fluorescence

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title = "Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies",

abstract = "Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.",

keywords = "Alkaline Phosphatase, Animals, Ascorbic Acid, Cattle, Kidney, Spectrometry, Fluorescence, Alkaline Phosphatase, Animals, Ascorbic Acid, Cattle, Kidney, Spectrometry, Fluorescence",

author = "Elisabetta Meucci and Alvaro Mordente",

year = "1983",

language = "English",

volume = "32",

pages = "231--238",

journal = "Italian Journal of Biochemistry",

issn = "0021-2938",

publisher = "Il Pensiero Scientifico",

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TY - JOUR

T1 - Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

AU - Meucci, Elisabetta

AU - Mordente, Alvaro

PY - 1983

Y1 - 1983

N2 - Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

AB - Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.

KW - Alkaline Phosphatase

KW - Animals

KW - Ascorbic Acid

KW - Cattle

KW - Kidney

KW - Spectrometry, Fluorescence

KW - Alkaline Phosphatase

KW - Animals

KW - Ascorbic Acid

KW - Cattle

KW - Kidney

KW - Spectrometry, Fluorescence

UR - http://hdl.handle.net/10807/9756

M3 - Article

VL - 32

SP - 231

EP - 238

JO - Italian Journal of Biochemistry

JF - Italian Journal of Biochemistry

SN - 0021-2938

ER -