Interaction between alkaline phosphatase and ascorbic acid by fluorescence and absorption studies

Ge Martorana, Elisabetta Meucci, Ga Miggiano, Alvaro Mordente, A. Castelli

Risultato della ricerca: Contributo in rivistaArticolo

Abstract

Very low amounts of ascorbic acid modify alkaline phosphatase fluorescence, absorption and enzymatic activity. A strong quenching of enzyme, tryptophan and tyrosine emission together with evident alterations of the protein absorption characteristics are observed. The catalytic activity inhibition probably reflects a perturbation of the active site environment due to the interaction of ascorbic acid with enzyme aminoacyl residues.
Lingua originaleInglese
pagine (da-a)231-238
Numero di pagine8
RivistaItalian Journal of Biochemistry
Volume32
Numero di pubblicazione4
Stato di pubblicazionePubblicato - 1983
Pubblicato esternamente

All Science Journal Classification (ASJC) codes

  • Biochimica

Keywords

  • Alkaline Phosphatase
  • Animals
  • Ascorbic Acid
  • Cattle
  • Fluorescence
  • Kidney
  • Spectrometry

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