Innate immune responses in bovine forestomachs are mediated by toll like receptors

Erminio Trevisi, Paolo Bani, L Turin, M Amadori, F. Riva

Risultato della ricerca: Contributo in libroContributo a convegno

Abstract

Interleukin-1/Toll Like Receptors (IL-1R/TLRs) play an important role in the inflammatory responses under a tight regulation. TIR8, also known as SIGIRR (Single Immunoglobulin IL-1R-Related molecule), is an orphan receptor belonging to the IL-1R/TLRs super-family, playing an important role in modulating the inflammatory response in the gastrointestinal tract in mice as an interleukin-1 (IL-1) receptor family antagonist. Previous studies demonstrated the active role of bovine forestomachs as key player in immune responses during alimentary disorders and inflammatory and infective processes in both the gastrointestinal (GI) tract and elsewhere. We demonstrated the potential of bovine forestomachs to receive, elaborate and produce signals and mediators of the innate immune response. In this respect, we showed the expression of TIR8, TLR4, IL-1beta, IL-10 and Caspase-1 in the forestomach wall, both by Real-Time PCR and Western blot. Furthermore, we demonstrated for the first time the presence of leukocytes and immune mediators, such as interferon gamma (IFN-gamma), in the rumen liquor. In particular, CD45-positive cells (by FACS and Real-Time PCR analysis) were demonstrated in the rumen content of fistulated and slaughtered cows. We also recovered from the ruminal content IFN-gamma that showed a moderate stability. All these points of evidence suggest an active role of the forestomachs in the immune modulation of the GI tract, but also during inflammatory disorders in other body compartments, such as uterus and joints. Forestomachs can receive signals and elaborate them, but they can also send signals to immune cells infiltrating the rumen content or other body compartments. Forestomachs have thus the capability to participate in a cross-talk with the immune system at both regional and systemic levels.
Lingua originaleEnglish
Titolo della pubblicazione ospiteActa of "2011 Joint annual meeting SIICA (Immunology Clinical Immunology and Allergology) and DGfI (German Sociey for Immunology)"
Pagine45
Numero di pagine1
Stato di pubblicazionePubblicato - 2011
Evento2011 Joint annual meeting SIICA (Immunology Clinical Immunology and Allergology) and DGfI (German Sociey for Immunology) - Riccione
Durata: 28 set 20111 ott 2011

Convegno

Convegno2011 Joint annual meeting SIICA (Immunology Clinical Immunology and Allergology) and DGfI (German Sociey for Immunology)
CittàRiccione
Periodo28/9/111/10/11

Keywords

  • immune response

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