TY - JOUR
T1 - Inflammosome in the human endometrium: further step in the evaluation of the ‘‘maternal side’’
AU - D'Ippolito, Silvia
AU - Tersigni, Chiara
AU - Marana, Riccardo
AU - Di Nicuolo, Fiorella
AU - Gaglione, Raffaele
AU - Rossi, Esther
AU - Castellani, Roberta
AU - Scambia, Giovanni
AU - Di Simone, Nicoletta
PY - 2016
Y1 - 2016
N2 - Objective: To investigate the expression of inflammosome components (NALP-3, associated speck-like protein containing a CARD
[ASC]) and their activation (caspase-1, interleukin [IL]-1b, and IL-18 secretion) in the human endometrium from fertile and women
with history of recurrent pregnancy loss (RPL).
Design: Experimental study.
Setting: University hospital.
Patient(s): Ten fertile women (control group [CTR]) and 30 women with RPL.
Intervention(s): None.
Main Outcome Measure(s): Endometrial samples were collected by hysteroscopy during the putative window of implantation and
evaluated for chronic endometrial inflammation by hystopathological analysis. Inflammosome expression was analysed by immunohystochemical
staining (27 RPL and 10 CTR women). The expression of NALP-3 and ASC protein was quantified by Western blot (30
RPL and 10 CTR women). Caspase-1 activation and IL-1b and IL-18 secretion was quantified by ELISA (30 RPL and 10 CTR women).
Result(s): We observed a significantly increased expression of inflammasome NALP-3 and ASC protein, an increased activation of
caspase-1, and increased levels of IL-1b and IL-18 in RPL endometrium compared with CTR.
Conclusion(s): Abnormal activation of endometrial innate immunity by means of inflammosome, stimulated by pathogen- or
damage-associated molecular patterns, may represent an additional mechanism, currently not investigated, negatively interfering
with endometrial receptivity. More studies are required [1] to identify the primary trigger of endometrial inflammosome activation
and its clinical impact in the occurrence of RPL; and [2] to validate the inflammosome
components as a novel family of endometrial biomarkers and promising therapeutic targets
in RPL
AB - Objective: To investigate the expression of inflammosome components (NALP-3, associated speck-like protein containing a CARD
[ASC]) and their activation (caspase-1, interleukin [IL]-1b, and IL-18 secretion) in the human endometrium from fertile and women
with history of recurrent pregnancy loss (RPL).
Design: Experimental study.
Setting: University hospital.
Patient(s): Ten fertile women (control group [CTR]) and 30 women with RPL.
Intervention(s): None.
Main Outcome Measure(s): Endometrial samples were collected by hysteroscopy during the putative window of implantation and
evaluated for chronic endometrial inflammation by hystopathological analysis. Inflammosome expression was analysed by immunohystochemical
staining (27 RPL and 10 CTR women). The expression of NALP-3 and ASC protein was quantified by Western blot (30
RPL and 10 CTR women). Caspase-1 activation and IL-1b and IL-18 secretion was quantified by ELISA (30 RPL and 10 CTR women).
Result(s): We observed a significantly increased expression of inflammasome NALP-3 and ASC protein, an increased activation of
caspase-1, and increased levels of IL-1b and IL-18 in RPL endometrium compared with CTR.
Conclusion(s): Abnormal activation of endometrial innate immunity by means of inflammosome, stimulated by pathogen- or
damage-associated molecular patterns, may represent an additional mechanism, currently not investigated, negatively interfering
with endometrial receptivity. More studies are required [1] to identify the primary trigger of endometrial inflammosome activation
and its clinical impact in the occurrence of RPL; and [2] to validate the inflammosome
components as a novel family of endometrial biomarkers and promising therapeutic targets
in RPL
KW - inflammasome
KW - inflammasome
UR - http://hdl.handle.net/10807/101671
U2 - 10.1016/j.fertnstert.2015.09.027
DO - 10.1016/j.fertnstert.2015.09.027
M3 - Article
SN - 0015-0282
VL - 105
SP - 111
EP - 118
JO - Fertility and Sterility
JF - Fertility and Sterility
ER -