In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via ¹⁴C-TdR labeling

Ornella Parolini, Cheng-Guang Wu, Ji-Chun Zhang, Cheng-Quan Xie, Antonietta Silini, Yi-Zhou Huang, Bing Lian, Min Zhang, Yong-Can Huang, Li Deng

Risultato della ricerca: Contributo in rivistaArticolo in rivista

11 Citazioni (Scopus)

Abstract

BACKGROUND: In order to shed light on the regenerative mechanism of mesenchymal stem cells (MSCs) in vivo, the bio-distribution profile of implanted cells using a stable and long-term tracking method is needed. We herein investigated the bio-distribution of human placental deciduas basalis derived MSCs (termed as PDB-MSCs) in nude mice after intravenous injection by carbon radioisotope labeling thymidine ((14)C-TdR), which is able to incorporate into new DNA strands during cell replication. RESULTS: The proliferation rate and radioactive emission of human PDB-MSCs after labeled with different concentrations of (14)C-TdR were measured. PDB-MSCs labeled with 1 μCi possessed high radioactivity, and the biological characteristics (i.e. morphology, colony forming ability, differentiation capabilities, karyotype and cell cycle) showed no significant changes after labeling. Thus, 1 μCi was the optimal concentration in this experimental design. In nude mice, 1 × 10(6) (14)C-TdR-labeled PDB-MSCs were injected intravenously and the organs were collected at days 1, 2, 3, 5, 30 and 180 after injection, respectively. Radiolabeled PDB-MSCs were found mainly in the lung, liver, spleen, stomach and left femur of the recipient nude mice at the whole observation period. CONCLUSIONS: This work provided solid evidence that (14)C-TdR labeling did not alter the biological characteristics of human placental MSCs, and that this labeling method has potential to decrease the signal from non-infused or dead cells for cell tracking. Therefore, this labeling technique can be utilized to quantify the infused cells after long-term follow-up in pre-clinical studies
Lingua originaleEnglish
pagine (da-a)55-63
Numero di pagine9
RivistaBMC Biotechnology
Volume15
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • Animals
  • Carbon Radioisotopes
  • Cell Tracking
  • Female
  • Humans
  • Mesenchymal Stromal Cells
  • Mice
  • Mice, Nude
  • Placenta
  • Pregnancy
  • Thymidine
  • Tissue Distribution

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