In vitro study on the inhibition of enzymatic systems in Italian and Carniolan honey bees by piperonyl butoxide new derivatives

Emanuele Mazzoni, Valeria Todeschini, Michela Panini, Olga Chiesa, Matteo Anaclerio, Hana Vinšová, Martin Kamler, Dalibor Titěra

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

4 Citazioni (Scopus)

Abstract

In this study we measured in vitro honey bee enzyme activity in presence of the synergist piperonyl butoxide (PBO) and some new benzodioxole and dihydrobenzofuran derivatives through absorbance and fluorescence spectrophotometric assays, to determine if and to what extent detoxification systems and acetylcholinesterases are affected by these compounds. Both Italian (Italy) and Carniolan (Czech Republic) honey bees were tested. In the case of Italian honey bees the esterase activity was partially but significantly inhibited by almost all the tested products. In Carniolan honey bees only the dihydrobenzofuran derivative EN16-41 significantly inhibited esterases. Other enzymatic systems, such as cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs), often involved in xenobiotics detoxification, as well as acetylcholinesterases (AchEs) seem not to be targeted by the studied synergists. These data seem to suggest that some of the investigated PBO analogues could not be detrimental to honey bees
Lingua originaleEnglish
pagine (da-a)237-244
Numero di pagine8
RivistaBulletin of Insectology
Volume70
Stato di pubblicazionePubblicato - 2017

Keywords

  • Apis mellifera
  • acetylcholinesterases
  • esterases
  • glutathione S-transferases
  • mixed function oxidases
  • piperonyl butoxide
  • synergists

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