In vitro rearing of Anagrus breviphragma (Hymenoptera: Mymaridae), an egg parasitoid of Cicadella viridis (Hemiptera: Cicadellidae), from second instar larva to adult on diets without insect components

Ilaria Negri, Elisabetta Chiappini, Maria Luisa Dindo, Luca Sighinolfi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

3 Citazioni (Scopus)

Abstract

We describe here the in vitro rearing of Anagrus breviphragma Soyka, an egg parasitoid of Cicadella viridis (Linnaeus), from the second instar larva to the adult stage. Three media, containing mainly either a commercial tissue culture medium (IPL-41), skimmed milk or veal homogenate, were tested. Larval development occurred on all the diets but few larvae reached the pupal stage on the diets containing skimmed milk or veal homogenate. On the tissue culture medium, pharate adults, albeit malformed, developed. Supplementing the tissue culture medium-based diet with chicken egg yolk alone, or combined with yeast extract, further improved parasitoid development. The addition of both egg yolk and yeast extract resulted in twelve out of twenty larvae reaching the adult stage, of which only four females emerged.
Lingua originaleEnglish
pagine (da-a)419-422
Numero di pagine4
RivistaEuropean Journal of Entomology
Volume101
Stato di pubblicazionePubblicato - 2004

Keywords

  • Anagrus breviphragma, Mymaridae, Cicadella viridis, egg parasitoid, rearing, artificial diets

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