TY - JOUR
T1 - In vitro Evaluation of the Cytotoxicity of Different Root Canal Filling Materials
AU - Lupi, Alessandro
AU - Giardina, Bruno
AU - Nocca, Giuseppina
PY - 2011
Y1 - 2011
N2 - Abstract
OBJECTIVE:
Aim of the present study was to evaluate the cytotoxicity of Real Seal 1 compared to other commercially available endodontic filling materials: Real Seal (SybronEndo, Orange, CA, USA) and Thermafil (Tulsa Dental, Tulsa, OK, USA).
MATERIAL AND METHODS:
Periodontal ligament cells from healthy patients were cultured. The eluate of Real Seal 1(TM) (RS1), Real Seal (RS) and Thermafil (TF) samples was used for the cells viability tests, both diluted (50%) or undiluted (100%). Incubation of the specimens was performed in culture medium for 24 h, 48 h and 72 h at 37 °C under sterile conditions. The cellular mortality was evaluated by MTT test. Results were statistically analysed and the statistical significance was set at p< 0.05.
RESULTS:
None of the studied materials showed toxic effects during the period of observation (0 -72 h) when compared to the control group. Only RS induced a very modest increase in cell mortality (about 3% at both concentrations used, during the first 24 hours), when increasing the incubation time, however, only the lower concentration continued to show modest toxicity.
CONCLUSIONS:
Results of the present study showed that all tested materials did not exhibit cytotoxic effects when compared to the control group
AB - Abstract
OBJECTIVE:
Aim of the present study was to evaluate the cytotoxicity of Real Seal 1 compared to other commercially available endodontic filling materials: Real Seal (SybronEndo, Orange, CA, USA) and Thermafil (Tulsa Dental, Tulsa, OK, USA).
MATERIAL AND METHODS:
Periodontal ligament cells from healthy patients were cultured. The eluate of Real Seal 1(TM) (RS1), Real Seal (RS) and Thermafil (TF) samples was used for the cells viability tests, both diluted (50%) or undiluted (100%). Incubation of the specimens was performed in culture medium for 24 h, 48 h and 72 h at 37 °C under sterile conditions. The cellular mortality was evaluated by MTT test. Results were statistically analysed and the statistical significance was set at p< 0.05.
RESULTS:
None of the studied materials showed toxic effects during the period of observation (0 -72 h) when compared to the control group. Only RS induced a very modest increase in cell mortality (about 3% at both concentrations used, during the first 24 hours), when increasing the incubation time, however, only the lower concentration continued to show modest toxicity.
CONCLUSIONS:
Results of the present study showed that all tested materials did not exhibit cytotoxic effects when compared to the control group
KW - biocompatibility
KW - biocompatibility
UR - http://hdl.handle.net/10807/4474
U2 - 10.2174/1874210601105010029
DO - 10.2174/1874210601105010029
M3 - Article
SN - 1874-2106
VL - 5
SP - 29
EP - 32
JO - THE OPEN DENTISTRY JOURNAL
JF - THE OPEN DENTISTRY JOURNAL
ER -