Impact of the different preparation methods to obtain autologous non‐activated platelet‐rich plasma (A‐PRP) and activated platelet‐rich plasma (AA‐PRP) in plastic surgery: Wound healing and hair regrowth evaluation

Simone Garcovich, Pietro Gentile, Claudio Calabrese, Barbara De Angelis, Laura Dionisi, Jacopo Pizzicannella, Ashutosh Kothari, Domenico De Fazio

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

16 Citazioni (Scopus)

Abstract

Autologous therapies using platelet‐rich plasma (PRP) need meticulous preparation—currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed‐Technique and Opened‐Technique) to isolate autologous human activated (AA‐PRP) or non‐activated PRP (A‐PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel®, Biomed®, Cascade® and Selphyl®, Mag‐18®, i‐Stem®, MyCells® and Regenlab®. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A‐PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A‐PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A‐PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed.
Lingua originaleEnglish
pagine (da-a)431-439
Numero di pagine9
RivistaInternational Journal of Molecular Sciences
Volume21
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • Activated PRP
  • Autologous platelet‐rich plasma
  • A‐PRP
  • Non‐activated PRP
  • PRP
  • PRP kit
  • PRP procedures
  • Platelet‐rich plasma
  • Regenerative plastic surgery

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