Identification and typing of the Candida parapsilosis complex: MALDI-TOF MS vs. AFLP

Brunella Posteraro, Maurizio Sanguinetti, Elena De Carolis, Antonietta Vella, Lambert A. M. Hensgens, Sonia Senesi, Arianna Tavanti

Risultato della ricerca: Contributo in rivistaArticolo in rivista

31 Citazioni (Scopus)

Abstract

In this study we compare the capability of amplification fragment-length polymorphism (AFLP) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify and subtype isolates of members of the Candida parapsilosis complex (C. parapsilosis, C. orthopsilosis, C. metapsilosis) and Lodderomyces elongisporus, which cannot be differentiated with biochemical methods. Both techniques correctly identified all isolates included in this study and clustered isolates within the different species. DNA-based and mass spectrum-based dendrograms yielded similar outcomes with regard to phylogenetic distance within C. orthopsilosis and C. parapsilosis species. However, a different clustering was obtained for C. metapsilosis for which AFLP was highly effective in differentiating. While MALDI-TOF MS was found to be a reliable method for species-level identification, further studies are required to assess its value as a fungal typing tool.
Lingua originaleEnglish
pagine (da-a)123-130
Numero di pagine8
RivistaMedical Mycology
Volume52
DOI
Stato di pubblicazionePubblicato - 2014

Keywords

  • AFLP
  • Amplified Fragment Length Polymorphism Analysis
  • Candida
  • Candida metapsilosis
  • Candida orthopsilosis
  • Candida parapsilosis
  • Cluster Analysis
  • Genotype
  • Humans
  • MALDI-TOF MS
  • Molecular Typing
  • Mycological Typing Techniques
  • Phenotype
  • Phylogeny
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • typing

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