Human hepatocytes in mice receiving pre -immune injection with human cord blood cells.

Paolo Turrini, Giovanni Monego, Jose Gonzalez, Sandra Cicuzza, Giuseppina Bonanno, Giovanni Zelano, Nadia Rosenthal, Giacomo Paonessa, Ralf Laufer, Julio Padron

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review


It is well established that certain subpopulations of human adult stem cells can generate hepatocyte-like cells when transplanted into adult immunosuppressed mice. In the present study, we wanted to explore whether xeno-transplantation of human cord blood CD34(+) (hCBCD34(+)) cells during pre-immune stages of development in immunocompetent mice might also lead to human-mouse liver chimerism. Freshly isolated hCBCD34(+) cells were xeno-transplanted into non-immunosuppressed mice by both intra-blastocyst and intra-fetal injections. One and four weeks after birth, immunostaining for different human-specific hepatocyte markers: human hepatocyte-specific antigen, human serum albumin, and human alpha-1-antitrypsin indicated the presence of human hepatocyte-like cells in the livers of transplanted animals. Detection of human albumin mRNA further corroborated the development of pre-immune human-mouse chimeras. The current report, besides providing new evidence of the potential of hCBCD34(+) cells to generate human hepatocyte-like cells, suggests novel strategies for generating immunocompetent mice harboring humanized liver.
Lingua originaleEnglish
pagine (da-a)66-73
Numero di pagine8
RivistaBiochemical and Biophysical Research Communications
Stato di pubblicazionePubblicato - 2005




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