TY - JOUR
T1 - Human cytomegalovirus-specific CD4(+) and CD8(+) T-cell response determination: comparison of short-term (24h) assays vs long-term (7-day) infected dendritic cell assay in the immunocompetent and the immunocompromised host
AU - Zelini, Paola
AU - Lilleri, Daniele
AU - Comolli, Giuditta
AU - Rognoni, Vanina
AU - Chiesa, Antonella
AU - Fornara, Chiara
AU - Locatelli, Franco
AU - Meloni, Federica
AU - Gerna, Giuseppe
PY - 2010
Y1 - 2010
N2 - Human cytomegalovirus (HCMV)-specific CD4(+) and CD8(+) T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV-infected dendritic cells (iDC), and two short-term (24 h) assays, one for CD4(+) stimulation by infected cell lysate (iCL), and the other for CD8(+) stimulation by a pool of 34 epitopic peptides (pep-pool). In the immunocompetent, the number of T-cells activated by either iCL or the pep-pool was significantly reduced with respect to iDC. In both SOTR and HSCTR, the number of T-cells activated by iDC was comparable to that activated by iCL or the pep-pool. A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay. (C) 2010 Elsevier Inc. All rights reserved.
AB - Human cytomegalovirus (HCMV)-specific CD4(+) and CD8(+) T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV-infected dendritic cells (iDC), and two short-term (24 h) assays, one for CD4(+) stimulation by infected cell lysate (iCL), and the other for CD8(+) stimulation by a pool of 34 epitopic peptides (pep-pool). In the immunocompetent, the number of T-cells activated by either iCL or the pep-pool was significantly reduced with respect to iDC. In both SOTR and HSCTR, the number of T-cells activated by iDC was comparable to that activated by iCL or the pep-pool. A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay. (C) 2010 Elsevier Inc. All rights reserved.
KW - Human cytomegalovirus
KW - T-cell response
KW - Infected dendritic cells
KW - Immunocompromised patients
KW - Epitopic peptide pool
KW - Immunocompetent host
KW - Infected cell lysate
KW - Human cytomegalovirus
KW - T-cell response
KW - Infected dendritic cells
KW - Immunocompromised patients
KW - Epitopic peptide pool
KW - Immunocompetent host
KW - Infected cell lysate
UR - http://hdl.handle.net/10807/250554
U2 - 10.1016/j.clim.2010.04.008
DO - 10.1016/j.clim.2010.04.008
M3 - Article
SN - 1521-6616
VL - 136
SP - 269
EP - 281
JO - Clinical Immunology
JF - Clinical Immunology
ER -