Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing

T. Ingegnere; F. R. Mariotti; A. Pelosi; C. Quintarelli; Angelis B. De; N. Tumino; F. Besi; C. Cantoni; Franco Locatelli; P. Vacca; L. Moretta

doi:10.3389/fimmu.2019.00957

Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing

T. Ingegnere
, F. R. Mariotti
, A. Pelosi
, C. Quintarelli
, Angelis B. De
, N. Tumino
, F. Besi
, C. Cantoni
, Franco Locatelli
, P. Vacca
, L. Moretta^*

^*Autore corrispondente per questo lavoro

IRCCS Ospedale pediatrico Bambino Gesù - Roma
University of Naples Federico II
University of Genoa

Risultato della ricerca: Contributo in rivista › Articolo

Abstract

CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Lingua originale	Inglese
pagine (da-a)	1-10
Numero di pagine	10
Rivista	Frontiers in Immunology
Volume	10
Numero di pubblicazione	APR
DOI	https://doi.org/10.3389/fimmu.2019.00957
Stato di pubblicazione	Pubblicato - 2019

OSS delle Nazioni Unite

Questo processo contribuisce al raggiungimento dei seguenti obiettivi di sviluppo sostenibile

SDG 3 Salute e benessere

All Science Journal Classification (ASJC) codes

Immunologia e Allergia
Immunologia

Keywords

Adoptive immunotherapy
Chemokine receptors
Chimeric Antigen Receptors
Electroporation
NK cells

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10.3389/fimmu.2019.00957

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abstract = "CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.",

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AU - Ingegnere, T.

AU - Mariotti, F. R.

AU - Pelosi, A.

AU - Quintarelli, C.

AU - De, Angelis B.

AU - Tumino, N.

AU - Besi, F.

AU - Cantoni, C.

AU - Locatelli, Franco

AU - Vacca, P.

AU - Moretta, L.

PY - 2019

Y1 - 2019

N2 - CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

AB - CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

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KW - Chemokine receptors

KW - Chimeric Antigen Receptors

KW - Electroporation

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KW - Adoptive immunotherapy

KW - Chemokine receptors

KW - Chimeric Antigen Receptors

KW - Electroporation

KW - NK cells

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Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing

Abstract

OSS delle Nazioni Unite

All Science Journal Classification (ASJC) codes

Keywords

Accesso al documento

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